Antimicrobial peptides

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai

Reexamination Certificate

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Details

C514S013800, C514S014800, C530S324000

Reexamination Certificate

active

07745390

ABSTRACT:
Artificial antimicrobial peptides are obtained by alterations in alpha helical portions of a known antimicrobial protein, granulysin. The peptides obtained have significantly improved antimicrobial activity and lack the ability to lyse mammalian cells, which may be toxic to a host. The peptides may be designed according to certain guidelines, and may further be modified by the addition of altered residues and alterations in normal peptide secondary and tertiary structure, including modifications in quaternary (multimeric) structure.

REFERENCES:
patent: 6485928 (2002-11-01), Stenger et al.
patent: WO 99/22755 (1999-05-01), None
patent: WO 2005/090385 (2005-09-01), None
Wang et. al., J. Immunol., 2000, vol. 165, pp. 1486-1490.
Zhuo Wang, et al., “Bactericidal and Tumoricidal Activities of Synthetic Peptides Derived from Granulysin,”The Journal of Immunology, 2000, 165: 1486-1490.
Anmei Deng, et al., “Granulysin, a Cytolytic Molecule, Is Also a Chemoattractant and proinflammatory activator,”The Journal of Immunology, 2005, 174: 5243-5248.
William A. Ernst, et al., “Granulysin, a T Cell Product, Kills bacteria by Altering Membrane Permeability”The Journal of Immunology, 2000, 165: 7102-7108.
Alan M. Krensky, et al., “Granulysin: A Novel Host Defense Molecule,”American Journal of Transplantation, Aug. 2005, 5: 1789-1792.
Qing LI, et al., “Hemolysis of Erythrocytes by Granulysin-Derived Peptides but Not by Granulysin,”Antimicrobial Agents and Chemotherapy, Jan. 2005, vol. 49, No. 1, 388-397.
Ayyalusamy Ramamoorthy, et al., “Cell selectivity correlates with membrane-specific interactions: A case study on the antimicrobial peptide G15 derived from granulysin,”Biochimica et Biophysica Acta1758:154-163, Mar. 2006.

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