Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1986-11-04
1989-09-26
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435810, 435947, 436518, 436531, 436533, 436534, 436536, 436543, 436808, 436811, 530350, 530806, 530808, 530822, 935 47, 935 59, C07K 1504, G01N 33531, G01N 33546, G01N 33569
Patent
active
048700061
ABSTRACT:
T. Cruzi polypeptide antigens that react with serum from chagasic individuals and does not cross-react with serum from uninfected individuals or individuals infected with related parasites such as Leishmania is described. The DNA from T. Cruzi culture trypomastigotes and epimastigotes coding for antigenic material having a molecular weight of 70 kd is identified, sequenced, and inserted into a cloning vector, which, in turn, is inserted into a host cell line. The expressed polypeptide is immunologically reactive with sera from Chagas' disease infect patients. The cloned gene for the 70 kd polypeptide is expressed and purified and a diagnostic test for Chagas' disease comprising the synthesized polypeptide is described.
REFERENCES:
patent: 3911097 (1975-10-01), Hanson
patent: 3993743 (1976-11-01), Hanson
Gonzales et al., Nucleic Acid Research, 13, 5789-5804, 1985.
Lizardi et al., Vaccines 85, Cold Spring Harbor Laboratory, pp. 67-70, 1985.
Chapman et al., Chem. Abstr., 101, 2173g, 1984.
Lanar et al., Biol. Abstr., 78, 67791, 1984.
Schechter et al., Infect. and Immun., 53, 547-552, Sep. 1986.
Snary et al., Biol. Abstr., 73, 61459, 1982.
Watson et al., Recombinant DNA, W. H. Freeman and Co., New York, 1983, pp. 86-87.
Dragon Elizabeth A.
Sias Stacey
Codon
Saunders David A.
Warden Robert J.
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