Antigenic fusionprotein carrying Galα1,3Gal epitopes

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...

Reexamination Certificate

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C530S300000, C530S403000, C530S415000, C530S387300

Reexamination Certificate

active

06943239

ABSTRACT:
The present invention relates to an antigenic fusionprotein, which carries multiple Galα1,3Gal epitopes. The fusion protein according to the invention may also be comprised of a heavily glycosylated mucin part, which mediates binding to selectins, such as PSGL-1, and a part, which exhibits immunoglobulin properties, such as the Fc part of IgG. The fusionprotein according to the invention is preferably used as an absorber to prevent a hyperacute rejection of a xenotransplant, such as a pig tissue or organ transplanted into a human patient. In addition, the invention relates to a method for the prevention of hyperacute rejection reaction in a patient who is to receive a xenotransplant.

REFERENCES:
patent: 5434131 (1995-07-01), Linsley et al.
patent: WO 94/10309 (1994-05-01), None
patent: WO 94/21799 (1994-09-01), None
patent: WO 97/07823 (1997-03-01), None
Sako, et al. 1993 Cell 1751 1179-1186, 1993.
Tsuni, et al 1990 Chem. Pharm. Bulletin (38) 765-768, 1990.
Godink, 1983 Monoclonal Abs :Principles & Practice p. 117, Table 4.2, 1983.
Kozlowski et al. Transplantation Proceedings, p. 961, 1997.
R. Rieben et al., “Detection, Immunoabsorption, and Inhibition of Cytotoxic Activity of Anti-alpha-Gal Antibodies Using Newly Developed Substances with Synthetic Gal alpha-1-3Gal Disaccharide Epitopes”, Dialog Information Services, file 5:Biosis Previews (R), Xenotransplantation 2 (2), 1995.
Mauro S. Sandrin et al., “Natural Human Anti-Galα(1,3) Gal Antibodies React with Human Mucin Peptides”, Glycoconjugate Journal (1997) 14:97-105, pp. 97-104.

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