Antigen recognized by patients with antibody associated paraneop

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4353201, 435325, 536 235, C12N 1500, C12N 1512

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active

058077057

ABSTRACT:
An isolated nucleic acid sequence encoding Hu paraneoplastic antigenic polypeptide is provided by this invention. This invention also provides a purified Hu antigenic polypeptide and compositions containing the purified Hu antigenic polypeptide. Further provided by this invention is a monoclonal antibody directed to an epitope on the Hu paraneoplastic antigenic polypeptide. Compositions containing this monoclonal antibody also are provided by this invention. This invention also provides methods of diagnosis and treatment using the compositions described hereinabove.

REFERENCES:
Brain, L. and Wilkinson, M. (1965) "Subacute cerebellar degeneration associated with neoplasma." Brain 88: 465-478 (Exhibit 2).
Dalmau, J., et al. (1990) "Detection of the anti-Hu antibody in the serum of patients with small cell lung cancer-A quantitative Western blot analysis." Ann. Neurol. 27: 544-552 (Exhibit 3).
Dalmau, J., et al. (1991) "Detection of the anti-Hu antibody in specific regions of the nervous system and tumor from patients with paraneoplastic encephalomyelitis/sensory neuronpathy." Neurology 41:1757-1764 (Exhibit 4).
Furneaux,, H.M., et al. (1990) "Autoantibody synthesis in the central nervous system of patients with paraneoplastic syndromes." Neurology 40: 1085-1091 (Exhibit 5).
Graus, F., et al. (1985) "Neuronal antinuclear antibody in sensory neuronopathy from lung cancer." Neurology 35:538-543 (Exhibit 6).
Graus, F., et al. (1987) "Neuronal antinuclear antibody (anti-Hu) in paraneoplastic encephalomyelitis stimulating acute polyneuritis." Acta Neurol. Scand. 75: 249-252 (Exhibit 7).
Posner, J.B. and Furneaux, H.M. (1990) "Paraneoplastic Syndromes." Immunologic Mechanisms in Neurologic and Psychiatric Disease (ed. B.H. Waksman, Raven Press, Ltd.) 187-219 (Exhibit 8).
Szabo, A., et al. (1991) "HuD, a paraneoplastic encephalomyelitis antigen, contains RNA-binding domains and is homologous to Elav and Sex-Lethal." Cell 67: 325-333 (Exhibit 9).

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