Antigen recognized by patients with antibody associated cerebell

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 723, 435 772, 435 79, 435 792, 436518, 436536, 436544, 436545, 436546, 436804, G01N 3353, G01N 33536

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active

059255269

ABSTRACT:
An isolated nucleic acid sequence encoding major Yo paraneoplastic antigenic polypeptide is provided by this invention. This invention also provides a purified major Yo antigenic polypeptide and compositions containing the purified major Yo antigenic polypeptide. Further provided by this invention is a monoclonal antibody directed to an epitope on the major Yo paraneoplastic antigenic polypeptide. Compositions containing this monoclonal antibody also are provided by this invention. This invention also provides methods of diagnosis and treatment using the compositions described hereinabove.

REFERENCES:
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Anderson, N.E., et al. (1988) "A variant of the anti-Purkinje cell antibody in a patient with paraneoplastic cerebellar degeneration." Neurology 38: 1018-1026.
Brain, L. and Wilkinson, M. (1965) "Subacute cerebellar degeneration associated with neoplasms." Brain 88: 465-478.
Darnell, R., et al. (1989) "Characterization of neural antigens recognized by autoantibodies in CSF and serum of a patient with cerebellar degeneration: co-expression in Purkinje cells and tumor lines of neuroectodermal origin." Neurology 39 (Supp.1): 385.
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Janson, J.-C. (1984) "Large-scale affinity purification-state of the art and future prospects." Trends in Biotechnology 2: 31-38.
Lampson, L. (1987) "Molecular bases of the immune response to neural antigens." Trends Neurosci. 10: 211-216.
Rodriguez, M., et al. (1988) "Autoimmune paraneoplastic cerebellar degeneration: ultrastructural localization of antibody-binding sites in Purkinje cells." Neurology 38: 1380-1386.
Vinson, C., et al. (1989) "Scissors-grip model for DNA recognition by a family of leucine zipper proteins." Science 246: 911-916.
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