Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1990-08-09
1993-02-09
Draper, Garnette D.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
530351, 53038822, 530399, 530395, 530827, 530857, 435 691, C07K 1500
Patent
active
051854309
ABSTRACT:
The present invention encompasses antigens recognized by the receptor protein, an antigen common to the surface of cells recognized and lysed by natural killer cells, monoclonal and heterologous antibodies which bind to the antigen proteins which are useful in their identification and purification, and methods for altering NK cell-mediated lysis of target cells.
The monoclonal antibodies (mAbs) of the present invention were prepared by cell fusions between spleen cells from mice immunized with NC-37 human lymphoblastoid B-cells, which are susceptible to lysis by NK cells, and myeloma cells.
The target cell antigen, a dimer of approximately 38,000 to 44,000 mw, seems to be evolutionarily conserved across species, from fish to mouse to man, an observation in agreement with the concept of NK cells being a primitive system, in terms of its appearance in evolution. The protein was purified from lysates of NC-37 cells by chromatography with mAbs bound to protein A beads followed by electrophoresis on 11% SDS-PAGE.
REFERENCES:
patent: 4607007 (1986-08-01), Lanier et al.
patent: 4734363 (1988-03-01), Dattagupta et al.
patent: 4739045 (1988-04-01), Odell
patent: 4767700 (1988-08-01), Wallace
Brooks, Immunobiology of Natural Killer Cells, vol. II, 179-200 (CRC Press, Inc. 1986).
Clayberger, et al., J. Immun. 136(5), 1537-1541 (Mar. 1, 1985).
Phillips, et al., Natural Immunity and Cell Growth Regulation, 3 1537-41 (Mar. 1986).
Werkmeister, et al., Cell. Immunology 80, 172-186 (1983).
Vodinelich, et al., Proc. Nat'l Acad. Sci (USA) 80, 835-839 (1983).
Dokhelar, et al., Eur. J. Immunol 14, 340-344 (1984).
Graves, et al., Dev. Comp. Immunol. 8, 293-302 (1984).
Evans, et al., Dev. Comp. Immunol 8, 303-312, 599-610, 823-833 (1984).
Evans, et al., Dev. Comp. Immunol. 11, 95-104 (1987).
Kornbluth, et al., J. Immunol. 134, 728-735.
Lanier, et al., Immun. Today 7, 132-134 (1986).
David, et al., J. Immunol. 138, 1831-2836 (1987).
Oldham, J. Biol. Response Modifiers 1, 217-231 (1982).
Brooks, et al., J. Immunol. 135, 1145-1152 (1985).
Carlson, Dev. and Comp. Immunol. 9 271-280 (1985).
Evans Donald L.
Harris David T.
Draper Garnette D.
University of Georgia Research Foundation Inc.
University of North Carolina
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