Antigen detection with affinity chromatography and parallel proc

Details Antigen detection with affinity chromatography and parallel proc Antigen detection with affinity chromatography and parallel proc

1993-12-27

1996-02-13

Saunders, David

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

2101982, 210656, 210659, 422 59, 422 69, 422 70, 4352872, 4352886, 436161, 436529, 436530, 436531, 436534, C01N 33543, C01N 33546

Patent

active

054910960

ABSTRACT:
A method and device for detecting the presence of a suspected antigen in a sample whereby the antigen is trapped using affinity chromatography and the remaining eluant is differentially analyzed against a parallel processed control. The sample is first chromatographically segregated into distinct zones and the resulting eluant is split into two streams. One stream is contacted with a solid phase having immobilized capture ligands specific to the antigen of interest. The other stream is passed through a similar solid phase where the capture ligands have been omitted. The two streams are then differentially analyzed, for example, with ultraviolet light absorption.

REFERENCES:
patent: 3458437 (1969-07-01), Ovans
patent: 3846074 (1972-03-01), Tulumello et al.
patent: 3923460 (1975-12-01), Parrott et al.
patent: 3981179 (1976-09-01), Roof
patent: 4016074 (1977-04-01), Porter
patent: 4137161 (1979-01-01), Shimada et al.
patent: 4551429 (1985-11-01), Freytag et al.
patent: 4879247 (1989-11-01), Ohlson
patent: 4895809 (1990-01-01), Schlabach et al.
patent: 4937200 (1990-06-01), Kumazawa et al.
patent: 4988447 (1991-01-01), Hellinger
patent: 4990250 (1991-02-01), Hellinger
patent: 5234586 (1993-08-01), Afeyan et al.
Journal of Chromatograph, 175 (1979) 269-281 Elsevier Scientific Publishing Company, Amsterdam, J. A. Fulton et al. "Dual-Detector-Post-Column Reactor System for the Detection of Isoenzymes Separated by High-Performance Liquid Chromatography".

Affiliated with

Also associated with

Rating

Antigen detection with affinity chromatography and parallel proc does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Antigen detection with affinity chromatography and parallel proc, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Antigen detection with affinity chromatography and parallel proc will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-240176