Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving fixed or stabilized – nonliving microorganism,...
Reexamination Certificate
2005-11-01
2005-11-01
Le, Long V. (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving fixed or stabilized, nonliving microorganism,...
C435S040520, C436S141000, C436S129000, C424S078200
Reexamination Certificate
active
06960450
ABSTRACT:
An object of the present invention is to provide a reagent for activating and unmasking antigens in a immuno-tissue-chemical staining. A slice of tissue cells on a slide glass is heated or heated under pressurization by an electric pot, autoclave, microwave oven, scalder, or constant-temperature bath for a sufficient time period in CCA aqueous solution of suitable pH and CCA concentration, thereby activating and unmasking antigenicity of antigen masked by aldehyde fixation.
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Shigeki Namimatsu et al; Reversing the Effects of Formalin Fixation with Citraconic Anhydride and Heat : A Universal Antigen Retrieval Method; Mar. 11, 2005; vol. 53(1); pp. 1-11; Journal of Histochemistry & Cytochemistry.
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Hamakawa Shinji: An investigation on antigen activation by heat treatment during enzyme-antibody staining using anti-CD4 monoclonal antibody (Byohri to Rinshoh (Pathology and Clinical Pathology)) 1999, 17(11): 1201-1205).
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Davis Deborah A.
Le Long V.
Shlesinger & Arkwright & Garvey LLP
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