Antifungal proteins, DNA coding therefore, and hosts...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

Reexamination Certificate

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Details

C435S091400, C435S183000, C435S025000, C800S279000, C530S350000, C536S023100, C536S023200, C536S023600

Reexamination Certificate

active

06864076

ABSTRACT:
The present invention provides an isolated protein obtainable from a plant source which has antifungal activity, specifically anti-Phytophthora activity and/or anti-Pythium activity and a molecular weight of about 55-65 kDa as judged by SDS PAGE-electrophoresis, an isolated DNA sequence comprising an open reading frame capable of encoding a protein according to the invention, preferably characterized in that it comprises an open reading frame which is capable of encoding a protein depicted in SEQ ID NO. 16, SEQ ID NO. 57, SEQ ID NO. 70, SEQ ID NO. 72 or SEQ ID NO. 74 or muteins thereof, and DNA capable of hybridizing therewith under stringent conditions. The invention further comprises plants incorporating chimeric DNA capable of encoding a protein according to the invention, and wherein the protein is expressed. Also shown is the carbohydrate and preferably hexose oxidating activity of said protein. Also methods are provided for combating fungi, especially Phytophthora and Pythium species, using a protein or a host cell capable of producing the protein.

REFERENCES:
patent: 9514784 (1995-06-01), None
patent: 9521924 (1995-08-01), None
patent: 9521929 (1995-08-01), None
patent: WO 9640935 (1996-12-01), None
Hu et al. Cloning and expression of a PR5-like from Arabidopsis: Inhibition of fungal growth by bacterially expressed protein, Plant Molecular Biology 34: 949-957, Aug. 1997.*
Woloshuk et al., Pathogen-Induced Proteins with Inhibitory Activity toward Phytophthora infestans, The Plant Cell 3: 619-628, Jun. 1991.*
Wu, G., et al.: “Disease Resistance Conferred by . . . in Transgenic Potato Plants” The Plant Cell, vol. 7, Oct. 1995,pp. 1357-1368, XP002065951.
Krivitzky, M. et al.: “The Arabidopsis Thaliana Transcribed Genome: The GDR CDNA Program” EMBL Sequence Data Library, Heidelberg, Germany, XP002025476: Accession No.: F19886, Mar. 1996.
Kay Kwang-AE Kim et al: “Glucose Oxidase as the Antifungal Principle of . . . Flavus” Canadian Journal of Microbiology: vol. 36, No. 11, Nov. 1990, pp. 760-764, XP000602906.
Dittrich, H. and Kutchan, T.M.: Molecular Cloning, Expression, and Induction of . . . of the , PNAS, vol. 88, Nov. 1991, pp. 9969-9973, XP002025475.

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