Antifungal preparations containing osmotin

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 691, 514 2, 530370, 800205, 800DIG42, 800DIG43, C12N 1500, C12P 2706, A61K 3800, C07K 1400

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active

058561510

ABSTRACT:
A method for inhibiting the growth and/or germination of a fungus that is susceptible to an osmotin protein by contacting the fungus, or causing the fungus to be contacted with, an effective amount of an osmotin protein. The fungus can be, for example, a plant pathogenic fungus, such as an Oomycete. In one embodiment of the invention, the effective amount of the osmotin protein is produced by a plant having incorporated into its genome a chimeric gene having a) an open reading frame encoding the osmotin protein, or a precursor of the osmotin protein, and having operably linked thereto b) a regulatory region which causes the chimeric gene to be expressed in the plant.

REFERENCES:
patent: 5389609 (1995-02-01), Woloshuk et al.
Singh et al. Molecular cloning of osmotin and regulation of its expression by ABA and adaptation to low water potential. Plant Physiology. 90:1096-1101, 1989.
Lund et al. Bacterial chitinase is modified and secreted in transgenic tobacco. Plant Physiology. 91:130-135, 1989.
Thaumtin-Like Pathogenesis--Related Proteins are Antifungal Alison J. Vigers, Plant Sci, 83(1992) 155-161.
A New Family of Plant Antifungal Proteins Alison J. Vigers, Molec. Plant--Microbe Interactions vol. 4 No. 4 pp. 315-323 1991.
Antifungal Activity of Tobacco Osmotin . . . Laura R. Abad, Plant Science 118 (1996) 11-23.
Roberts et al, "Isolation and Partial Characterization of Two Anti-Fungal Proteins From Barley", Biochimica et Biophysica Acta 880(1986) 161-170.
Dayhoff et al, Protein Segment Dictionary pp. v-vii and 1 (1978).
Lipman et al, "Rapid and Sensitive Protein Similarity Searches", Science vol. 227, pp. 1435-1441 (1985).

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