Antifungal polypeptides

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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C530S300000, C530S324000, C435S069100, C514S012200

Reexamination Certificate

active

07598346

ABSTRACT:
The invention relates to compositions including amino acid sequences isolated from fungal fermentation broths and methods for protecting a plant from a pathogen, particularly a fungal pathogen.

REFERENCES:
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patent: 2004/0214272 (2004-10-01), LaRosa et al.
patent: WO 98/27805 (1998-07-01), None
patent: WO 02/090384 (2002-11-01), None
patent: WO 2004/048421 (2004-06-01), None
patent: WO 2006/014316 (2006-02-01), None
patent: WO 2006/014316 (2006-02-01), None
Galgóczy, László et al., “Sensitivity of Different Zygomycetes to thePencillium chrysogenumAntifungal Protein (PAF),”J. of Basic Microbiology, 2005, pp. 136-141, vol. 45, No. 2.
Horvath, Henriette et al., “Genetically Engineered Stem Rust Resistance in Barley Using the Rpg1 Gene” Jan. 7, 2003, Proceedings of the National Academy of Sciences of USA, National Academy of Science, Washington, DC, US, pp. 364-369, vol. 100, No. 1.
Theis, T. et al., “New Insights into the Target Site and Mode of Action of the Antifungal Protein ofAspergillus giganteus,” Research in Microbiology, Elsevier, Amsterdam, NL, Jan. 2005, pp. 47-56, vol. 156, No. 1.
Leiter, Eva et al., “Antifungal Protein PAF Severely Affects theIintegrity of the Plasma Membrane ofAspergillus nidulansand Induces an Apoptosis-like Phenotype,”Antimicrobial Agents and Chemotherapy, Jun. 2005, pp. 2445-2453, vol. 49, No. 6.
Dewan, M.M., and K. Sivasithamparam, “Occurrence of Species ofAspergillusandPenicillumin Roots of Wheat and Ryegrass and Their Effect on Root Rot Caused byGaeumannomyces graminisvar.Tritici,” Aust. J. Bot., 1988, pp. 701-710, vol. 36.
Reboux, G., “Mycotoxines: Health Effects and Relationship to Other Organic Compounds,”Revue Francaise D'Allergologie et D'Immunologie Clinique2006, pp. 208-212, vol. 46(3).
Yuen, G.Y., “Interactions ofPseudomonas fluorescensStrain E6 with Ornamental Plants and Its Effect on the Composition of Root-Colonizing Microflora,”Phytopathology, 1986, pp. 176-180, vol. 76(2).
Falcón-Perez, J.M. et al., “Functional Domain Analysis of the Yeast ABC Transporter Ycflp by Site-directed Mutagenesis,”J. Biol. Chem., 1999, pp. 23584-23590, vol. 274.
Hill, et al., “Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase FromEscherichia coli,” Biochem. Biophys. Res., 1998, pp. 573-577, vol. 244.
Lazar, et al., “Tranforming Growth Factor α: Mutation of Aspartic Acid 47 and Leucine 48 Results in Different Biological Activities,”Mol. Cell. Biol., 1988, pp. 1247-1252, vol. 8.
Lee, D.G., et al., “Isolation and Characterization of a Novel Antifungal Peptide fromAspergillus niger,” Biochemical and Biophysical Research Communications, 1999, pp. 646-651, vol. 263.
Marcus, J.P., et al., Purification, Characterization and cDNA Cloning of an Antimicrobial Peptide FromMacadamia integrifolia, Eur. J. Biochem.1997, pp. 743-749, vol. 244.
Veronese, et al., “In Defense Against Pathogens. Both Plant Sentinels and Foot Soldiers Need to Now the Enemy,”Plant Physiology, 2003, pp. 1580-1590, vol. 131.
Rey, M, et al., “Unexpected Homology Between Inducible Cell Wall Protein QID74 of Filamentous Fungi and BR3 Salivary Protein of the InsectChironomus,” Proc. Natl. Acad. Sci. USA, 1998, pp. 6212-6216, vol. 95.
The USPTO Publication Site for Issued and Published Sequences (PSIPS), XP-002442732, SEQ ID No. 346842 for US 20040214272.
Database EMBL, Jan. 2, 2004, EBI Accession No. EMBL:CF453104, “wt—2—018Fusarium verticillioideswild type substraction libraryGibberella moniliformiscDNA, mRNA sequence”, XP002442733.
Database EMBL, Jul. 12, 2001, EBI Accession No. EMBL:BI200659, “o1a11fs.r1Fusarium sporotrichioidesTri 10 overexpressed cDNA libraryFusarium sporotrichioidescDNA clone o1a11fs 5′ mRNA sequence”, XP002442734.
Database EMBL, Jun. 2, 2004, EBI Accession No. EBML:CN811083, Fg03—12c23—R, Fg03)AAFC—ECORC—Fusarium—graminearum—mycelium—trichothecene—prodcutr ionGibberella zeaecDNA clone Fg03—12c23, mRNA sequence, XP002442735.

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