Antifungal polypeptides

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide confers pathogen or pest resistance

Reexamination Certificate

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C536S023740, C800S288000

Reexamination Certificate

active

07923599

ABSTRACT:
Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

REFERENCES:
patent: 7319087 (2008-01-01), Hansen
patent: WO 02/090384 (2002-11-01), None
patent: WO 04/001012 (2003-12-01), None
Sequence alignment.
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Lazar, et al., “Transforming Growth Factor α: Mutation of Aspartic Acid 47 and Leucine 48 Results in Different Biological Activities,”Mol. Cell. Biol., 1988, pp. 1247-1252, vol. 8.
Lee, D.G., et al., “Isolation and Characterization of a Novel Antifungal Peptide fromAspergillus niger,” Biochemical and Biophysical Research Communications, 1999, pp. 646-651, vol. 263.
Marcus, J.P., et al., Purification, Characterization and cDNA Cloning of an Antimicrobial Peptide FromMacadamia integrifolia, Eur. J. Biochem. 1997, pp. 743-749, vol. 244.
Veronese, et al., “In Defense Against Pathogens. Both Plant Sentinels and Foot Soldiers Need to Now the Enemy,”Plant Physiology, 2003, pp. 1580-1590, vol. 131.
Database Uniprot [Online], Nov. 1, 1996 “Antifungal Properties”, Database Accession No. Q01701.

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