Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Patent
1997-12-08
1998-12-01
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
252308, 252309, 252320, 252321, 252351, 252358, 95155, 435 41, 435 713, 435183, 435243, 435812, C12N 100, B01D 1904, C12P 1304, C07C 5143
Patent
active
058437347
DESCRIPTION:
BRIEF SUMMARY
This application claims the benefit under 35 U.S.C. .sctn.371 of prior PCT International Application No. PCT/EP96/02357 which has an International filing data of May 31, 1996, published as WO97/00942, Jan. 9, 1997, which designated the United States of America, the entire contents of which are hereby incorporated by reference.
FIELD OF THE INVENTION
This invention relates to an antifoaming agent for fermentation. More specifically, this invention relates to an antifoaming agent for fermentation, which has both foam-breaking effects and foam-inhibiting effects and is therefore excellent in defoaming effects, and does not adversely affect the fermentation production.
BACKGROUND OF THE INVENTION
In the conventional fermentation industry, for example, in the production of useful substances by deep-aerobic culture, a large amount of foams is formed, which causes various problems. Described specifically, a fermenter is filled with foams, which lowers a culturing capacity per unit volume and causes the culture solution to overflow from the fermenter. There is accordingly a demand for overcoming such a problem.
To suppress the formation of such foams, it is the common practice to add an antifoaming agent to a medium. As such an antifoaming agent, polyoxyalkylene polyhydric alcohol ethers, polyoxyalkylene alkyl ethers, polyoxyalkylene fatty acid esters, polyoxyalkylene alkyl ether fatty acid esters and the like are known (Japanese Patent Laid-Open Nos. 4282/1975, 121482/1975, 135298/1979, 169583/1981 and 35073/1990). In addition, antifoaming agents which break foams as soon as they are formed and thus immediately exhibit defoaming effects are also known (Japanese Patent Laid-Open No. 54680/1994).
These conventional antifoaming agents for fermentation, however, have not both the foam-breaking effects for breaking foams and foam-inhibiting effects for inhibiting the formation of foams from a liquid surface so that their defoaming effects are not sufficient. The use of an antifoaming agent having excellent foam-breaking effects and that having excellent foam-inhibiting effects in combination, on the other hand, offset their effects each other, leading to a problem that neither effect can be obtained. In addition, these antifoaming agents are accompanied with the problem that they inhibit the growth of microorganisms and production of target products, thus exerting a bad influence on the fermentation production.
An object of the present invention is therefore to provide an antifoaming agent for fermentation, which has both foam-breaking effects and foam-inhibiting effects and therefore has excellent defoaming effects, and does not adversely affect the fermentation production.
DISCLOSURE OF THE INVENTION
In view of the foregoing circumstances, the present inventors have conducted an extensive investigation. As a result, it has been found that an antifoaming agent for fermentation which is excellent in both foam-breaking effects and foam-inhibiting effects and does not adversely affect the fermentation production can be obtained by the use of a reaction product, which has been obtained by adding a predetermined amount of ethylene oxide and propylene oxide to a mixture of an oil or fat and a polyhydric alcohol, and a specific compound in combination, leading to the completion of the invention.
In one aspect of this invention, there is thus provided an antifoaming agent for fermentation, which comprises the following components (A) and (B):
(A) a reaction product obtained by the addition polymerization of 50 to 250 moles in total of ethylene oxide and propylene oxide (the molar ratio of ethylene oxide to propylene oxide=1:1 to 1:4) to 1 mole of a mixture composed of an oil or fat and a polyhydric alcohol containing at least three hydroxyl groups; and
(B) at least one compound selected from the group consisting of:
In another aspect of this invention, there is also provided a process for the fermentation production of an amino acid, a carboxylic acid, an enzyme or an antibiotic, which comprises adding an antifoamin
REFERENCES:
patent: 5567606 (1996-10-01), Hayashi et al.
Hasebe Keiko
Hayashi Masaharu
Shonaka Masafumi
Kao Corporation
Lilling Herbert J.
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