Antienzyme termination in enzyme immunoassays

Details Antienzyme termination in enzyme immunoassays Antienzyme termination in enzyme immunoassays

1980-06-02

1982-07-27

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435184, 435810, 23230B, 424 12, G01W 3354, C12W 999

Patent

active

043418660

ABSTRACT:
An improved method is provided for performing an enzyme immunoassay, where the signal producing compound is chemically labile. Particularly, in homogeneous enzyme immunoassays, where the enzyme is conjugated with a hapten, and an antibody binding to hapten reduces or inhibits the activity of the enzyme-hapten conjugate, the immunoassay is performed in accordance with conventional procedures. After a sufficient time, antienzyme is added which rapidly deactivates the enzyme, so that the signal remains constant for long periods of time.

REFERENCES:
patent: 3875011 (1975-04-01), Rubenstein et al.
patent: 4233401 (1980-11-01), Yoshida et al.
patent: 4234680 (1980-11-01), Hevey et al.

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