Antibody to and assay for peptide linked-pyridinoline as indicat

Details Antibody to and assay for peptide linked-pyridinoline as indicat Antibody to and assay for peptide linked-pyridinoline as indicat

1997-06-27

1999-11-23

Ceperley, Mary E.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is inorganic

436530, 436815, 5303889, 5303898, 5303911, 5303913, 530809, 935 95, 935110, G01N 33553, C07K 1681

Patent

active

059899255

ABSTRACT:
A monoclonal antibody, Serex A93, recognizes peptide linked pyridinoline in Type I bone collagen fragments and can be used to quantitate bone resorption. The hybridoma producing this antibody has been deposited with the American Type Culture Collection, Rockville, Md., under accession number HB-12254. The epitope reactive with the antibody is stable to acid hydrolysis and therefore is not a linear peptide. The antibody recognizes pyridinoline in bound or conjugated form, but not in the free form found in urine. The antibody is useful in immunoassays for determining bone resorption, utilizing body fluids such as urine, saliva, blood, or sweat.

REFERENCES:
patent: 4973666 (1990-11-01), Eyre
patent: 5300434 (1994-04-01), Eyre
patent: 5320970 (1994-06-01), Eyre
patent: 5527715 (1996-06-01), Kung et al.
patent: 5620861 (1997-04-01), Cerelli et al.
patent: 5661039 (1997-08-01), Kung et al.
Bailey, A. J. et al., "Age related changes in the reducible cross-links of collagen," FEBS Lett. 16(2):86-88 (1971).
Banes, A. J., et al., "Nonmineralized and Mineralized Compartments of Bone: The Role of Pyridinoline in Nonmineralized Collagen," Biochem. Biophys. Res. Commun. 113(3):975-981 (1983).
Barnes, M. J. et al., "Age-Related Variations in Hydroxylation of Lysine and Proline in Collagen," Biochem. J. 139(2):461-468 (1974).
Eyre, D. R., "Crosslink Maturation in Bone Collagen," in The Chemistry and Biology of Mineralized Connective Tissues, pp. 51-55, (Veis, A. ed.) (Elsevier, New York, 1981).
Eyre, D. R., "Crosslink Maturation in Bone Collagen," Develop. Biochem. 22:50-55 (1981).
Eyre, D.R. et al., "Quantitation of Hydroxypyridinium Crosslinks in Collagen by High-Performance Liquid Chromatography," Analyt. Biochem. 137:380-388 (1984).
Eyre, D. R. In: The Chemistry and Biology of Mineralized Tissues, p. 105, (Butler, W. T. ed.) (Ebsco Media Inc., Birmingham, Ala., 1985).
Eyre, D. R. et al., "Cross-linking in Collagen and Elastin," Ann. Rev. Biochem. 53:717-748 (1984).
Fujimoto, D. et al., "Isolation and Characterization of a Fluorescent Material in Bovine Achilles Tendon Collagen," Biochem. Biophys. Res. Commun. 76(4):1124-1129 (1977).
Gunja-Smith and Boucek, "Collagen cross-linking compounds in human urine," Biochem J. 197(3):759-762 (1981).
Robins, S. P., "An enzyme-linked immunoassay for the collagen cross-link pyridilone," Biochem J. 207(3):617-620 (1982).
Robins et al., "Measurement of the cross linking compound, pyridinoline, in urine as an index of collagen degradation in joint disease," Ann. Rheum. Diseases 45:969-973 (1986).
Singer, F. R., et al., "Paget's Disease of Bone," in Metabolic Bone Disease, vol. II, pp. 489-575, (eds. Avioli, L. V. and Krane, S. M.) (Academic Press, New York, 1978).
Walters, C. et al., "Collagen Crosslinks in Human Dentin: Increasing Content of Hydroxypyridinium Residues with Age," Calc. Tiss. Intl. 35:401-405 (1983).

Affiliated with

Also associated with

Rating

Antibody to and assay for peptide linked-pyridinoline as indicat does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Antibody to and assay for peptide linked-pyridinoline as indicat, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Antibody to and assay for peptide linked-pyridinoline as indicat will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1220877