Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-06-07
1999-04-20
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
5303871, 5303882, 53038825, 53038885, 5303891, 435 72, 435 721, 435 79, C07K 1618, G01N 33536, G01N 33537, G01N 33541
Patent
active
058957464
ABSTRACT:
Aminoterminal propeptide of type I procollagen exists in serum in two forms: classical type I procollagen which is a heterotrimer containing two pro .alpha.1-chains and one pro .alpha.2-chain of type I procollagen, and an .alpha.1-homotrimer type I procollagen containing three identical pro .alpha.1-chains. These intact, trimeric aminoterminal propeptides may be isolated without the use of proteolytic enzymes and the resultant propeptides may be used to prepare antibodies specific for the intact trimeric propeptide, having no affinity for the monomeric form of the propeptide. Such antibodies are useful in methods of assaying intact trimeric aminoterminal propeptide of type I procollagen in serum, without false information resulting from inadvertent assay of the monomeric form of the propeptide.
REFERENCES:
Frei, Hepatology 4:830-834, 1984.
Rohde, Presenius 7. Anal-Chem. 290:151-152,1978.
Taubman, Science 186:1115-1117, 1974.
Pesciotta, Biochemistry 19:2447-2454, 1980.
Foellmer, Eur J. Biochem 134:183-189, 1983.
Eberling, Peter R. et al., "Utility of Type I Procollagen Propeptide Assays for Assessing Abnormalities in Metabolic Bone Diseases", Journal of Bone and Mineral Research, vol. 7, No.11, pp. 1243-1250 (1992).
Jukkola, Arja, et al., "Procollagen Synthesis and Extracellular Matrix Deposition in MG-63 Osteosarcoma Cells", Journal of Bone and Mineral Research, vol. 8, No. 6, pp. 651-657 (1993).
Kohler, G., et al., "Continuous cultures of fused cells secreting antibody of predefined specificity", Nature, vol. 256, pp. 495-497 (1975).
Linkhart, Susan G., et al., "Synthetic Peptide-Based Immunoassay for Amino-Terminal Propeptide of Type I Procollagen: Application for Evaluation of Bone Formation", Clin. Chem., vol. 39, No. 11, pp. 2254-2258 (1993).
Mellko, Jukka et al., "Radioimmunoassay of the Carboxyterminal Propeptide of Human Type I Procollagen", Clin. Chem., vol. 36, No. 7, pp. 1328-1332 (1990).
Price, Karen Mo., et al., "Development of a radioimmunoassay for fetal antigen 2", Clin. Chim. Acta., vol. 224, pp. 95-102 (1994).
Skerra, Arne, et al., "Assembly of a Functional Immunoglobulin F.sub.v Fragment in Escherichia coli", Science, vol. 240, pp. 1038-1041 (1988).
Teisner, B., et al., "Fetal antigen 2: An amniotic protein identified as the aminopropeptide of the .alpha.1 chain of human procollagen type I", APMIS, vol. 100, pp. 1106-1114 (1992).
Kauppila Saila
Melkko Jukka
Risteli Juha
Risteli Leila
Johnson Nancy A.
Orion-yhtyma Oy
Scheiner Toni R.
LandOfFree
Antibody to aminoterminal propeptide of type 1 procollagen, and does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Antibody to aminoterminal propeptide of type 1 procollagen, and , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Antibody to aminoterminal propeptide of type 1 procollagen, and will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2247536