Antibody steric hindrance immunoassay with two antibodies

Details Antibody steric hindrance immunoassay with two antibodies Antibody steric hindrance immunoassay with two antibodies

1973-12-17

1976-01-27

Monacell, A. Louis

Textiles: fiber preparation

23230B, 424 12, 424 1, G01n 3100, C12k 104

Patent

active

039350746

ABSTRACT:
A novel immunoassay is provided, as well as particular reagents, for determining the presence of a ligand. A Reagent is employed having at least two epitopes, one of the epitopes being common with the ligand, and the other epitopes being foreign to the ligand. The two epitopes are positioned in the Reagent so that antibody bound to one of the epitopes sterically inhibits the binding of antibody to the second epitope.
In carrying out the assay, the sample, the Reagent, and antibodies to the two epitopes are combined. Because of the steric inhibition to the simultaneous binding of the two antibodies to the Reagent, the amount of antibody bound to the epitope of the Reagent foreign to the ligand will be related to the amount of ligand present in the assay medium. By analyzing directly or indirectly for the antibody bound to the epitope foreign to the ligand, and comparing the results to known standards, qualitative or quantitative determinations of the amount of ligand may be made. Various detector systems may be employed for determining the amount of antibody to the foreign epitope which is unbound or bound. These systems include stable free radicals, enzymes, radioactive labels, fluorescers, and the like.

REFERENCES:
patent: 3839153 (1974-10-01), Wilhelmus et al.
patent: 3852157 (1974-12-01), Rubenstein et al.

Affiliated with

Also associated with

Rating

Antibody steric hindrance immunoassay with two antibodies does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Antibody steric hindrance immunoassay with two antibodies, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Antibody steric hindrance immunoassay with two antibodies will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1810199