Liquid purification or separation – Processes – Chromatography
Patent
1980-01-28
1981-04-28
Adee, John
Liquid purification or separation
Processes
Chromatography
210927, B01D 1508
Patent
active
042644496
ABSTRACT:
An antibody-specific solid phase immunoadsorbent is prepared by adsorptively binding a lipid-containing viral antigen, such as hepatitis B surface antigen, to the surface of a porous glass carrier having an average pore diameter within the range of about 40-200 nm and an adsorptive surface which within the pH range of 2-8 is capable of selectively and stably binding lipid-containing materials, employing an adsorption buffer having a pH within such range. The high adsorptive selectivity of the carrier surface for the antigen under such conditions, enables preparation of the immunoadsorbent directly from whole human plasma containing the antigen, without the necessity for prior antigen purification. The resultant immunoadsorbent has a high complexing specificity, efficiency and capacity for the specific antibody to the bound antigen, is capable of providing a reasonably high yield of the specific antibody, and is stable under the conditions required for antibody-antigen complexing and dissociation, thereby rendering it highly effective for the isolation and purification of the specific antibody by affinity chromatography techniques.
A specific application of the methods and materials described which is of particular clinical significance is in providing a relatively simple and inexpensive procedure for recovering purified hepatitis B antibody from standard immune serum globulin.
REFERENCES:
patent: 4029583 (1977-06-01), Chang et al.
patent: 4111838 (1978-09-01), Schaeffer
patent: 4162355 (1979-07-01), Tsibais
Adee John
American National Red Cross
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