Antibody recognizing proliferative human liver cells,...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Reexamination Certificate

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C530S388200, C435S007200, C424S141100, C424S152100

Reexamination Certificate

active

10509096

ABSTRACT:
The invention provides a monoclonal antibody which specifically recognizes proliferative human hepatocytes, a method for isolating proliferative human hepatocytes by using the antibody, and a method for inducing to differentiate the proliferative human hepatocytes to functional human hepatocytes. Further, the invention provides the proliferative human hepatocytes and functional human hepatocytes obtained by the methods, as well as a cell kit and a hybrid artificial liver comprising those hepatocytes.

REFERENCES:
patent: 682106 (1996-05-01), None
patent: 61-189299 (1986-08-01), None
patent: 00/43498 (2000-07-01), None
patent: 00/03001 (2001-01-01), None
Flodby et al., “Increased Hepatic Cell Proliferation and Lung Abnormalities in Mice Deficient in CCAAT/Enhancer Binding Protein alpha,” Oct. 1996, Journal of Biological Chemistry, 271(40): 24753-24760.
Hilan et al. 1996. Journal of Hepatology 24:385-390.
anti-Ki-monoclonal antibody information from Zymed (http://www.biocompare.com/itemdetails.asp?itemid=86403&catid=3194).
Hillman et al (1996, Journal of Hepatology, 24:385-390).
Zymed information sheet for anti-ki-67 monoclonal antibody.
R. Jover et al., “Cytochrome P450 regulation by hepatocyte nuclear factor 4 in human hepatocytes: a study using adenovirus-mediated antisense targeting”, Hepatology, vol. 33, No. 3, pp. 668-675, 2001.
D. Stroup et al., “HNF4 and COUP-TFII interact to modukate transcription of the cholesterol 7 alpha-hydroxylase gene (CYP7A1)”, J. Lipid Res., vol. 41, No. 1, pp. 1-11, 2000.
S. Harish et al., “Transcriptional activation by hepatocyte nuclear factor-4 in a cell-free system derived from rat liver nucleic”, Nucleic Acids Res., vol. 29, No. 5, pp. 1047-1053, 2001.

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