Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-04-10
1999-06-01
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 71, 435 792, 436518, 436528, 436548, 436811, 5303882, 5303911, 935110, G01N 33543, G01N 33577, G01N 3368
Patent
active
059087579
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
1. Field
The present invention relates to an antibody reagent for detecting dissecting aortic aneurysm (recently also called aortic dissection) which comprises an antibody to a smooth muscle myosin heavy chain, and use of the antibody reagent.
2. Description of Related Art
Dissecting aortic aneurysm is a disease with severe chest pains and is caused by disruption of the aortic media by blood entering through a laceration of the luminal vascular wall. Dissecting aortic aneurysm is caused in aorta in most cases but is caused also in branches in some cases. As causes for the disease, there are suggested not only the degeneration and weakening of the intima (e.g. cystic medionecrosis and arterio-sclerosis) but also the extension of aorta, hypertension, etc.
A typical example of disease with chest pains is acute myocardial infarction. Acute myocardial infarction may be diagnosed without much difficulty through electrocardiograph change or biochemical blood testing. By contrast, in the case of dissecting aortic aneurysm, a specific change is hardly observed in an electrocardiograph or blood testing in spite of the high lethality of this disease. Therefore, diagnosis of this disease requires extreme care.
As a method for diagnosis of dissecting aortic aneurysm, echo examination, CT (X-rays computed tomography), DSA (digital subtraction angiography), MRI (magnetic resonance imaging), etc. have been attempted and have produced rather good results (Common Disease Series 4: Angina Pectoris.multidot.Myocardial Infarction, pp. 310-313, Nankodo K. K.). All of these methods, however, require special equipment, and hence are not always satisfactory as a method for use in an urgent examination wherein the method is required to be always able to be carried out anywhere.
Accordingly, the present invention is mainly intended to provide a method for detecting dissecting aortic aneurysm which is applicable to an urgent examination, and a reagent for use in the method.
SUMMARY OF THE INVENTION
The present inventors conducted detailed researches with the expectation that, in the cases of a disease associated with blood vessel disturbance such as dissecting aortic aneurysm, a substance constituting the blood vessel is released into blood. Consequently, the present inventors concluded that the blood vessel disturbance can be diagnosed by detecting smooth muscle myosin heavy chain which is a main protein constituting the blood vessel.
Since smooth muscle myosin heavy chain is biochemically different from skeletal muscle myosin heavy chain, cardiac muscle myosin heavy chain and non-muscle myosin heavy chain, it is relatively easy to obtain an antibody specific for smooth muscle myosin heavy chain. Therefore, an antibody to smooth muscle myosin heavy chain was prepared, whereby there has been established an assay system which enables detection of smooth muscle myosin heavy chain in blood. The smooth muscle myosin heavy chain levels in the sera of healthy individuals were measured by this assay system and found to be low. On the other hand, as a result of measurement in the sera of patients with dissecting aortic aneurysm, there were observed significantly higher smooth muscle myosin heavy chain levels which clearly reflect the clinical symptoms of the patients. Thus, it was confirmed that measuring smooth muscle myosin heavy chain in blood is useful for detecting dissecting aortic aneurysm, whereby the present invention has been accomplished.
Accordingly, the present invention relates to an antibody reagent for detecting dissecting aortic aneurysm which comprises an antibody to smooth muscle myosin heavy chain.
The present invention also relates to a kit for detecting dissecting aortic aneurysm which comprises an antibody reagent to smooth muscle myosin heavy chain and a washing solution containing a surfactant.
The present invention further relates to a method for detecting dissecting aortic aneurysm which comprises measuring smooth muscle myosin heavy chain in a sample, and detecting dissecting aortic
REFERENCES:
Sartore et al., 1989. Myosin heavy-chain isoforms in human smooth muscle. Eur. J. Biochem. 179: 79-85.
Mohammad et al., 1989. The distribution of heavy-chain isoforms of myosin in airways smooth muscle from adult and neonate humans. Biochem. J. 260: 421-426.
Schneider et al., 1985. Localization and topography of antigenic domains within the heavy chain of smooth muscle myosin. J. Cell Biol. 101: 66-72.
Chemical Abstract of JP 5-148160, Chem Abstracts 119: 383, #119:176770r.
Toru Suzuki et al., "Novel Biochemical Diagnostic Method for Aortic Dissection", Biochemical Diagnosis of Aortic Dissection, Circulation, vol. 93, No. 6, pp. 1244-1249, Mar. 15, 1996.
Hirohisa Katoh et al., "A Novel Immunoassay of Smooth Muscle Myosin Heavy Chain in Serum", Journal of Immunological Methods, vol. 185, pp. 57-63, 1995.
Katoh Hirohisa
Nagai Ryozo
Grun James L.
Housel James C.
Yamasa Corporation
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