Antibody reactive to hamster immunoglobulins and assay using sam

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 79, 4351722, 436513, 436536, 436543, 436548, G01N 33535

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active

052328325

ABSTRACT:
A monoclonal antibody specifically reactive to hamster immunoglobulins, a hybridoma producing said monoclonal antibody, and an immunoassay utilizing said monoclonal antibody are provided. The monoclonal antibody is useful in an immunoassay as a secondary antibody to a hamster-derived primary antibody or antiserum raised against various antigens.

REFERENCES:
Leptin et al-Chem. Abst. vol. 101 (1984) p. 88588K.
Bird et al-Chem. Abst. vol. 101 (1984) p. 22827m.
S. Bright et al, "Generation and Characterization of Hamster-Mouse Hybridomas Secreting Monoclonal Antibodies With Specificity for Lipopolysaccharide Receptor", The Journal of Immunology, vol. 145, No. 1, Jul. 1, 1990, Baltimore, pp. 1-7.
G. Kohler et al, "Continuous Cultures of Fused Cells Secreting Antibody of Predefined Specificity", Nature, vol. 256, Aug. 7, 1975, London, pp. 495-497.
T. Kayano et al, "Growth of Rat-Mouse Hybridoma Cells in Immunosuppressed Hamsters, An Easy and Effective Method to Prepare Monoclonal Antibodies from heterohybridoma Cell Lines", Journal of Immunological Methods, vol. 130, No. 1, 1990, Amsterdam, The Netherlands, pp. 25-31.
T. Springer et al, "Monoclonal Antibodies Specific for Rat IgG1, IgG2a, and IgG2b Subclases, and Kappa Chain Monotypic and Allotypic Determinants: Reagents for Use With Rat Monoclonal Antibodies", Hybridoma, vol. 1, No. 3, 1982, New York, pp. 257-273.

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