Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1990-05-25
1992-05-05
Moezie, F. T.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
5303871, 530412, 530415, 530416, 5303881, 5303892, C07K 312, C07K 322
Patent
active
051109131
ABSTRACT:
Method of purifying a protein from a solution of substances comprising the steps of passing the solution through an ion exchange material at a pH which facilitates binding of the protein of interest, washing the bound protein at a different pH at which the bound protein does not elute but which the free protein would not bind to the ion exchange material, and then eluting the protein at a pH which facilitates elution. Method is especially useful in purification of antibodies such as antibodies to tumor necrosis factor.
REFERENCES:
patent: 4234570 (1980-11-01), Kanbayashi et al.
patent: 4771128 (1988-09-01), Ferris et al.
Yost et al., Practical Liquid Chrometography-An Introduction, (Perlin-Elmes, Corp.) pp. 113-115 (1980).
Gill, Electrophoresis, vol. 6, pp. 282-286 (1985).
Fagerstam et al., J. Chromatog. vol. 266, pp. 523-532 (1983).
Marston, Biochem. J., vol. 240, pp. 1-12 (1986).
Coan Michael
Lee Vivian W.
Enayati Elizabeth F.
Giblin James A.
Miles Inc.
Moezie F. T.
Rozycki Andrew G.
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