Antibody purification

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C530S387300, C530S387700, C530S388220, C530S388800, C530S388850

Reexamination Certificate

active

07038017

ABSTRACT:
A process for purifying an antibody is provided. In this process, a mixture containing the antibody and a contaminant is subjected to low pH hydrophobic interaction chromatography (LPHIC) at low salt concentration. The antibody is eluted from the column in the fraction which does not bind thereto. This process can be preceded and followed by other purification steps.

REFERENCES:
patent: 4000098 (1976-12-01), Hofstee
patent: 5429746 (1995-07-01), Shadle et al.
patent: 5591828 (1997-01-01), Bosslet et al.
patent: 5648237 (1997-07-01), Carter
patent: 5747035 (1998-05-01), Presta et al.
patent: 6054297 (2000-04-01), Carter et al.
patent: 6066719 (2000-05-01), Zapata
patent: B-57621/90 (1991-01-01), None
patent: WO 89/06692 (1989-07-01), None
patent: WO 92/12993 (1992-08-01), None
patent: WO 92/22653 (1992-12-01), None
patent: WO 93/11161 (1993-06-01), None
patent: WO 94/13804 (1994-06-01), None
patent: WO 94/13806 (1994-06-01), None
patent: WO 95/22389 (1995-08-01), None
patent: WO 97/14719 (1997-04-01), None
Shalaby et al J. Exp. Med 175:217-225, 1992.
Abe et al., “Purification of monoclonal antibodies with light-chain heterogeneity produced by mouse hybridomas raised with NS-1 myelomas: application of hydrophobic interaction high-performance liquid chromatography”Journal of Biochemical and Biophysical Methods27:215-227 (1993).
Alfthan et al., “Purification of Labelled Antibodies by Hydrophobic Interaction Chromatography”J. Chromatography470 (385-389) : 385-389 (1989).
Axelsson K., “Bacterial Lipopolysaccharides and Glutathione Mixed Disulfides as Possible Contaminants of Human Growth Hormone Produced with the Use ofE. coliK12”Acta Chemica Scandinavica, Series B39(1) : 69-77 (1985).
Berkowitz et al., “Use of high-performance hydrophobic interaction chromatography for the determination of salting-out conditions of proteins”Journal of Chromatography389 : 317-321 (1987).
Bridonneau et al., “Behaviour of human Immunoglobulin G subclasses on thiophilic gels: comparison with hydrophobic interaction chromatography”Journal of Chromatography616 :197-204 (1993).
Bywater et al., “Description of Immuglobulins from Protein A-Sepharose Cl-4B under Mild Conditions”Journal of Immunological Methods64 : 1-6 (1983).
Carter et al., “High level Escherichia coli expression and production of a bivalent humanized antibody fragment”Bio/Technology10 : 163-167 (1992).
Cruse et al.Illustrated Dictionary of Immunology, CRC Press, Inc. pps. 18-19, 107-109 (1995).
Danielsson et al., “One-step purification of monoclonal IgG antibodies from mouse ascites”Journal of Immunological Methods115: 79-88 (1988).
Eigenbrot et al., “X-Ray Structures of Fragments From Binding and Nonbinding Versions of a Humanized Anti-CD18 Antibody : Structural Indications of the Key Role of VHResidues 59 to 65”Proteins: Structure, Function, and Genetics18:49-62 (1994).
Fausnaugh et al., “Solute and Mobil Phase Contributions to Retention in Hydrophobic Interaction Chromatography of Proteins”J. Chromatography359 :131-146 (1986).
Fendly et al., “The Extracellular Domain of HER2
eu Is a Potential Immunogen for Active Specific Immunotherapy of Breast Cancer”Journal of Biological Response Modifiers9:449-455 (1990).
Gagnon et al., “Method Development and Scale-Up in the Purification of Monoclonal Antibodies by Ion Exchange and Hydrophobic Interaction Chromatography”Abstract of the Annual Meeting(Abstract No. 0-4) (May 13, 1990).
Hildreth et al., “A Human Lymhocyte-associated Antigen Involved in Cell-mediated Lympholysis”European Journal of Immunology13:202-208 (1983).
Holliger et al., ““Diabodies”: Small bivalent and bispecific antibody fragments”Proc. Natl. Acad. Sci. USA90:6444-6448 (Jul. 1993).
Hudziak et al., “p185HER2Monoclonal Antibody Has Antiproliferative Effects In Vitro and Sensitizes Human Breast Tumor Cells to Tumor Necrosis Factor”Molecular & Cellular Biology9(3) : 1165-1172 (Mar. 1989.
Inouye et al., “Fragmentation of mouse monoclonal antibodies of the immunoglobulin M class into F(ab')2fragments and the application of the fragments to immunoassays”Protein Engineering(Abstract No. 44) pps. 1018-1019 (1993).
Inouye et al., “One-Step Purification of F(ab')2Fragments of Mouse Monoclonal Antibodies (immunoglobulins G1 and M) by Hydrophobic Interaction HPLC”Animal Cell Technology: Basic & Applied Aspects5:609-616 (1993).
Inouye et al., “Single-step purification of F(ab')2μfragments of mouse monoclonal antibodies (immunoglobulins M) by hydrophobic interaction high-performance liquid chromatography using TSKgel Ether-5PW”Journal of Biochemical and Biophysical Methods26:27-39 (1993).
Johansson et al., “Purification of proteins by salt promoted adsorption chromatography”Biol. Recombinant Microorg. Anim. Cells(Oholo 34 Meeting) pps. 409-414 (1991).
Kelley et al., “Antigen Binding thermodynamics and Antiproliferative Effects of Chimeric and Humanized anti-p18HER2 Antibody Fab Fragments”Biochemistry31:5434-5441 (1992).
Kurzban et al., “Purification of Bovine Liver Rhodanese by Low-pH Column Chromatography”Protein Expression and Purification2:279-384 (1991).
Mendelson et al., “Preparation and Characterization of Polyclonal and Monoclonal Antibodies against Human Aromatase Cytochrome P-450 (P-450AROM), and Their Use in its Purification”Archives of Biochemistry & Biphysics243 (2) :480-491 (1985).
Morimoto et al., “Single-step purification of F(ab')2fragments of mouse monoclonal antibodies (immunoglobulins G1) by hydrophobic interaction high performance liquid chromatography using TSKgel Phenyl-5PW”Journal of Biochemical and Biophysical Methods24:107-117 (1992).
Nardella et al., “Nonimmunospecific Protein-Protein Interactions of IcG: Studies of the Binding of IgG to IgG Immunoadsorbents”Journal of Immunology120(3): 739-744 (1978).
Nau et al., “The Role of Hydrophobic Interaction Chromotography in Antibody Purification—Optimization of Mobile Phase Conditions”BioChromatography5(2) :62-73 (1990).
Neblock et al., “Conjugation and Evaluation of 7E3 X P4B6, a chemically Cross-Linked Bispecific F9ab'2 Antibody which Inhibits Platelet Aggregation and Localizes Tissue Plasminogen Activator to the Platelet Surface”Bioconjugate Chem3:126-131 (1992).
Panka et al., “Variable region framework differences result in decreased or increased affinity of variant anti-digoxin antibodies”Proc. Natl. Acad. Sci. USA85:3080-3084 (May 1988).
Paulus, H., “ Preparation and Biomedical Applications of Bispecific Antibodies”Behring Inst. Mitt.78:118-132 (1985).
Pavlu et al., “Rapid Purification of Monoclonal Antibodies by High-Performance Liquid Chromatography”Journal of Chromatography359:449-460 (1986).
Rea et al., “The Rapid Development of Hydrophobic Interaction Chromatography Purification of a Murine Monoclonal IgG2aF(ab')2Fragment ”Journal of Cellular Biochemistry(Abstract No. X1-206) Suppl. A:pg. 50 (1993).
Regnier, F., “High-Performance Liquid Chromatography of Biopolymers”Science222(4621) :245-252 (1983).
Rodrigues et al., “Development of a Humanized Disultide-stablized Anti-p185HER2 Fv-B-Lactamase Fusion Protein for Activation of a Cephalosporin Doxorubicin Prodrug”Cancer Research55:63-70 (Jan. 1, 1995).
Rodrigues et al., “Engineering Fab'Fragments for Efficient F(ab)2Formation inEscherichia coliand for Improved In Vivo Stability”The Journal of Immunology151(12) : 6954-6961 (Dec. 15, 1993).
Rudikoff et al., “Single amino acid substitution altering antigen-binding specificity”Proc. Natl. Acad. Sci, USA79:1979-1983 (1982).
Scopes, “Protein Purification: Principles and Practice ”Springer Advanced Texts in Chemistry, Springer Advanced Texts in Chem

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Antibody purification does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Antibody purification, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Antibody purification will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3648153

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.