Antibody for the detection and quantification of atrazine

Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal

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260112R, 260121, 424 85, 435 7, 436532, 436819, 436547, C07G 700

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active

045307865

ABSTRACT:
This invention relates to an antibody capable of both specifically detecting and quantifying minute quantities of the herbicide, atrazine, left in biological samples, such antibody having been made by the process of first substituting a soluble straight chain amino acid at the 4 or 6 position of 2-chloro-4(isopropylamino)-6-(ethylamino)-S-atrazine so as to leave the chlorine exposed at the 2 position along with one of the amino groups, then conjugating the resulting substitution product at the site of the amino acid group to a lysine-rich protein, using the mixed anhydride method in a strongly-basic aqueous solution, then inoculating a susceptible host with the antigen to evoke an immune response, and thereafter harvesting the antibody.

REFERENCES:
patent: 3557073 (1971-01-01), Kay
patent: 3619371 (1971-11-01), Crook et al.
patent: 3674767 (1972-07-01), Lilly et al.
patent: 3852157 (1974-12-01), Rubenstein et al.
patent: 4007089 (1977-02-01), Smith, III
patent: 4275160 (1981-06-01), Singh et al.
patent: 4288553 (1981-09-01), Singh et al.
patent: 4357311 (1982-11-01), Schutt

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