Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1986-03-26
1988-10-25
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
424 858, 436532, 436547, 436815, 436819, 530363, 530367, 530387, 530405, 530806, 530807, G01N 3353, G01N 33549, C07K 1514
Patent
active
047804084
ABSTRACT:
An antibody specific to trifluralin can be produced by first substituting a soluble, straight chain amino acid for a propyl group of .alpha.,.alpha., .alpha.-trifluoro-2,6-dinitro-N-N-dipropyl-p-toluidine. The trifluoro group at the 4 position, the nitro group at the 2 position and the other nitro group at the 6 position are left exposed. Thereafter, the resulting substitution product is conjugated, at the site of the substituted amino acid group, with a lysine-rich protein. The resulting compound is then used as an antigen to evoke an immune response in a host animal or antibody producing cell line. The antibody produced against the antigen is then harvested and used in an assay such as a radioimmunoassay or enzyme linked immunosorbant assay (ELISA) to determine the presence and concentrations of trifluralin in agriculturally significant substances such as plant materials, soil and water or to otherwise test for trifluralin contamination of water, food and air.
REFERENCES:
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patent: 4275160 (1981-06-01), Singh et al.
patent: 4530786 (1985-07-01), Dunbar et al.
Chemical Abstracts, vol. 77, 1972, Abstract No. 71229g, Lavy et al.
Chemical Abstracts, vol 99, 1983, Abstract No. 117692n, Helmuth et al.
Chemical Abstracts, vol. 98, 1983, Abstract No. 174431e, Benigni et al.
Dunbar Bohn D.
Hudson James M.
Niswender Gordon D.
Colorado State University Research Foundation
Hoffer Florina B.
Warden Robert J.
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