Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Patent
1990-08-29
1993-03-02
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
4351885, C12N 900, C12P 1320
Patent
active
051908654
ABSTRACT:
Hydrolyses of various chiral compounds are performed in stereospecific manner by antibodies generated from analogs of transition states of the compounds. The compounds susceptible to such hydrolysis include esters, thioesters and amides. In certain embodiments of the invention, antibodies with a strong selectivity toward one of the two enantiomers with respect to the chiral substrate are generated from haptens which is a mixture of enantiomers. A method of screening antibodies thus generated for those which are active towards the desired cleavage is also disclosed, using a test compound similar in structure to the intended substrate but which contains both a fluorescence donor group and a fluorescence quenching group, situated on opposite sides of the cleavage site. Cleavage by active antibodies results in separation of the groups and a resulting termination of the quenching effect.
REFERENCES:
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Massey, R. J. (1987) Nature 328, 457-458.
K. D. Janda et al., Science 244:437-440 (28 Apr. 1989).
S. J. Benkovic et al., Proc. Natl. Acad. Sci. U.S.A. 85:5355-5358 (1988).
S. J. Pollack et al., J. Am. Chem. Soc. 111:5961-2 (1989).
P. G. Schultz et al., Chemical and Engineering News, May 28, 1990, pp. 26-40.
N. Nishino et al., J. Biol. Chem. 255(8):3482-3486 (1980).
J. M. Brown et al., Nature 342:631-636 (7 Dec. 1989).
Patterson Jr. Charles L.
The Regents of the University of California
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