Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide
Patent
1998-09-02
2000-08-01
Guzo, David
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using a micro-organism to make a protein or polypeptide
435 79, 5303871, 5303891, 5303911, 5303913, G01N 3353
Patent
active
060965223
ABSTRACT:
This invention provides isolated nucleic acid encoding human MAD2, isolated human MAD2 protein. This invention further provides a method of detecting the presence of MAD2 in a tissue sample, a method of determining whether a tumor is susceptible to treatment with a mitotic spindle inhibitor by detecting the presence of MAD2 in the tumor and a method of suppressing tumor formation in a subject which comprises administering the nucleic acid encoding human MAD2 to the subject in an amount effective to enhance expression of MAD2. This invention also provides a nucleic acid reagent capable of detecting the MAD2 gene or gene product and a method for in situ identification of tumors which may be susceptible to treatment with mitotic spindle inhibitors by detecting the absence of nucleic acid encoding MAD2 in the tumor.
REFERENCES:
patent: 5834298 (1998-11-01), Benezra
Armstrong S.A., et al. (1993) "cDNA Cloning and Expression of the .alpha. and .beta. Subunits of Rat Rab Geranylgeranyl Transferase," J. Biolog. Chem. 268(16):12221-12229.
Brown, M.S. and Goldstein, J.L. (1993) "Mad Bet for Rab," Nature 366:14-15.
Jiang, Y., et al. (1993) "Bet2p and Mad2p are Components of a Prenyltransferase that Adds Geranylgeranyl onto Ypt1p and Sec4,"p Nature 366:84-86.
Li, R., et al. (1993) "The Mitotic Feedback Control Gene MAD2 Encodes the .alpha.-Subunit of a Prenyltransferase," Nature 366:82-84.
Travers, C. et al. (1989) "Rapid Screening of A Human Genomic Library In Yeast Artificial Chromosomes For A Single-Copy Sequences," Proc. Natl. Acad. Sci. 89:5898-5092.
Woods, D. et al. (1980) The Construction Of A Recombinant cDNA Library Representative Of the Poly(A).sup.+ mRNA Population From Normal Human Lymphocytes.
Sigma Catalog (1992) Molecular Biology Products D5037, D6537, D3035 and D3160.
Gura, T. (1995) "Antisense Has Growing Pains," Science 270:575-577.
Fassler, R. et al. (1995) "Knockout Mice: How To Make Them And Why. The Immunological Approach," Int. Arch. Allergy Immunol. 106:323-334.
Mullins, J. et al. (1993) "Transgenesis In Nonmurine Species," Hypertension 22(4):630-633.
Orkin, S. et al. (1995) "Report And Recommendations Of The Panel To Assess The NIH Investment In Research On Gene Therapy," distributed by the National Institutes Of Health, Bethesda, Maryland.
Lombardo, C. et al. (1994) ".beta.-II-Spectrin (Fodrin) and .beta.-I.SIGMA.2-Spectrin (Muscle) Contain NH.sub.2 -and COOH-terminal Membrane Association Domains (MAD1 and MAD2)," J. Biol. Chem. 269(46):29212-29219.
Guzo David
Larson Thomas G.
Sloan-Kettering Institute for Cancer Research
White John P.
LandOfFree
Antibody and method for detecting the human homolog of MAD2 does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Antibody and method for detecting the human homolog of MAD2, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Antibody and method for detecting the human homolog of MAD2 will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-662505