Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-06-07
1998-02-10
Feisee, Lila
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 721, 435 74, 435 77, 5303881, 53038822, 5303913, 5303871, C07K 1600, G01N 3353, G01N 33567, G01N 33573
Patent
active
057167885
ABSTRACT:
One shortcoming of methotrexate chemotherapy is that previously responsive tumors can become refractory to methotrexate after continued exposure. Such methotrexate resistance may be due to underexpression of reduced folate carrier (RFC) protein. The present invention provides DNA molecules encoding human RFC. The present invention also relates to expression vectors comprising RFC-encoding DNA molecules, and to the use of such vectors to restore methotrexate sensitivity in mammalian cells. The present invention further relates to antibodies that bind with human RFC protein, and to methods of detecting human RFC protein using such antibodies.
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Cowan Kenneth H.
Dixon Kathy
He Rui
Moscow Jeffrey A.
Ebert Ray F.
Feisee Lila
The United States of America as represented by the Department of
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