Antibodies to complexes of ligand receptors and ligands and thei

Details Antibodies to complexes of ligand receptors and ligands and thei Antibodies to complexes of ligand receptors and ligands and thei

1993-06-01

1996-01-02

Woodward, Michael P.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 435 79, 435 792, 435 794, 435971, 436501, 436514, C12Q 168, G01N 3353, G01N 33531, G01N 33577

Patent

active

054807921

ABSTRACT:
Methods and test devices for detecting the presence or amount of target ligand in non-competitive sandwich ligand-receptor assay processes. Antibodies which bind to the complex of ligand receptor and target ligand but do not bind significantly to the ligand receptor and which bind the target ligand with substantially less affinity than the complex are taught and their uses described. These assays can be used to eliminate the "hook" effect in non-competitive sandwich assays. Furthermore, the antibodies are selected and assay methods described so that, as a result of the assay process, no detectable response is observed due to the binding of antibody and ligand receptor in the absence of target ligand.

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