Antibodies to aggrecan-degrading metalloprotease and...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Reexamination Certificate

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C530S389100, C435S007100, C435S007400

Reexamination Certificate

active

07049412

ABSTRACT:
The invention is directed to the family of aggrecan degrading metallo proteases (ADMPs) that exhibit the ability to cleave the aggrecan core protein between amino acid residues Glu373-Ala374. The invention encompasses the nucleic acids encoding such enzymes, processes for production of recombinant ADMPs, compositions containing such enzymes, and the use of these enzymes in various assays and for the development of novel inhibitors for use as therapies for diseases involving aggrecanase-mediated degradation of cartilage or other aggrecanase-associated diseases.

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J D Sandy, J Westling, R D Kenagy, M L Iruela-Arispe, C Verscharen, J C Rodriguez-Mezaneque, D R Zimmermann, J M Lemire, J W Fischer, T N Wight, A W Clowes, “Versican V1 Proteolysis in Human Aorta in vivo occurs at the Glu441-Ala442 Bond, a Site That is Cleaved by Recombinant ADAMTS-1 and ADAMTS-4”, J. Biol. Chem. 276. (16); 13372-13378, Apr. 20, 2001.
M A. Pratta, M D. Tortorella, and E C Arner “Age-related Changes in Aggrecan Glycosylation Affect Cleavage by Aggrecanase” J. Biol. Chem. 275 (50): 39096-39102. Dec. 15, 2000.
JD Sandy, J Westling, RD Kenagy, ML Iruela-Arispe, C Verscharen, JC Rodriguez-Mezaneque, DR Zimmerman, JM Lemire, JW Fischer, TN Wright, AW Clowes, “Versican V1 proteolysis in human aorta in vivo occurs at the Glu441-Ala442 bond, a site that is cleaved by recombinant ADAMTS-1 and ADAMTS-4”, J. Biol. Chem. 275 (50): 39096-39102. Dec. 15, 2000.
M.A. PRATTA, M.D. TORTORELLA, AND E C ARNER “Age-related Changes in Aggrecan Glycosylation Affect Cleavage by Aggrecanase”J. Biol. Chem. 275 (50): 39096-39102. Dec. 15, 2000.

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