Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Reexamination Certificate
2010-05-28
2011-12-06
Gamett, Daniel C (Department: 1647)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
C530S389100, C530S388100, C530S388240, C424S184100
Reexamination Certificate
active
08071733
ABSTRACT:
The present invention provides two novel polypeptides, referred to as the “N” and “C” fragments of hedgehog, or N-terminal and C-terminal fragments, respectively, which are derived after specific cleavage at a G′CF site recognized by the autoproteolytic domain in the native protein. Also included are sterol-modified hedgehog polypeptides and functional fragments thereof. Methods of identifying compositions which affect hedgehog activity based on inhibition of cholesterol modification of hedgehog protein are described.
REFERENCES:
patent: 5143830 (1992-09-01), Holland et al.
patent: 5789543 (1998-08-01), Ingham et al.
patent: 5844079 (1998-12-01), Ingham et al.
patent: 0 020 029 (1980-12-01), None
patent: WO 96/16668 (1996-06-01), None
Clarke and Armstrong, “Cellular Lipid Binding Proteins: Expression, Function, and Nutritional Regulation,”FASEB J. (1989), 3:2480-2487.
Echelard et al., “Sonic Hedgehog, a Member of a Family of Putative Signaling Molecules, is Implicated in the Regulation of CNS Polarity,”Cell(1993), 75:1417-1430, Cell Press.
Ericson et al., “Two Critical Periods of Sonic Hedgehog Signaling Required for the Specification of Motor Neuron Identity,”Cell(1996), 87:661-673, Cell Press.
Farese, Jr. and Herz, “Cholesterol Metabolism and Embryogenesis,”TIG(1998), 14(3):115 -120.
Ganong, William F., “Review of Medical Physiology,” A Lange Medical Book, (1995) 273, 438, Appleton & Lange, Norwalk, Connecticut.
Hammerschmidt et al., “The World According toHedgehog,” TIG. (1997), 13(1):14-15, Elsevier Science Ltd.
Hynes et al., “Induction of Midbrain Dopaminergic Neurons by Sonic Hedgehog,”Neuron(1995), 15:35-44.
Ingham, Philip W., “Signalling by Hedgehog Family Proteins inDrosophilaand Vertebrate Development,”Curr. Opin. Genetics Dev. (1995), 5:492-498, Current Biology Ltd.
Kolf-Clauw et al., “Abnormal Cholesterol Biosynthesis as in Smith-Lemli-Opitz Syndrome Disrupts Normal Skeletal Development in the Rat,”J. Lab. Clin. Med. (1998), 131:222-227, Mosby, Inc.
Krauss et al., “A Functionally Conserved Homolog of the Drosophila Segment Polarity Gene hh is Expressed in Tissues with Polarizing Activity in Zebrafish Embryos,”Cell(1993), 75:1431-1444, Cell Press.
Lange and Steck, “Cholesterol Homeostasis,”J. Biol. Chem. (1994), 269(47):29371-29374, The American Society for Biochemistry and Molecular Biology, Inc.
Lee et al., “Secretion and Localized Transcription Suggest a Role in Positional Signaling for Products of the Segmentation Genehedgehog,” Cell(1992), 71:33-50, Cell Press.
Lee et al., “Autoproteolysis inhedgehogProtein Biogenesis,” Science (1994), 266:1528-1537.
Mohler and Vani, “Molecular Organization and Embryonic Expression of thehedgehogGene Involved in Cell-Cell Communication in Segmental Patterning ofDrosophila,” Development(1992), 115:957-971.
Ngo et al., “Computational Complexity, Protein Structure Prediction, and the Levinthal Paradox,” In Merz, Jr. and Le Grand (eds.),The Protein Folding Problem and Tertiary Structure Prediction(1994), 433, 492-495, Birkhauser Boston.
Placzek et al., “Induction of Floor Plate Differentiation by Contact-Dependent, Homeogenetic Signals,”Development(1993), 117:205-218, The Company of Biologists Limited.
Porter et al., “Cholesterol Modification of Hedgehog Signaling Proteins in Animal Development,”Science(1996), 274:255-259.
Porter et al., “Hedgehog Patterning Activity: Role of a Lipophilic Modification Mediated by the Carbodxy-Terminal Autoprocessing Domain,”Cell(1996), 86:21-34, Cell Press.
Prickett et al., “A Calcium-Dependent Antibody for Identification and Purificaiton of Recombinant Proteins,”Biotechniques(1989), 7(6):580-589.
Reeck et al., ““Homology” in Proteins and Nucleic Acids: A Terminology Muddle and a Way Out of It,”Cell(1987), 50:667, Cell Press.
Tabata and Kornberg, Hedgehog is a Signaling Protein with a Key Role in Patterning Drosophila Imaginal “Discs,”Cell(1994), 76:89-102, Cell Press.
Beachy Philip A.
Moon Randall T.
Porter Jeffrey A.
DLA Piper (LLP) US
Gamett Daniel C
The Johns Hopkins University School of Medicine
University of Washington
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