Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Reexamination Certificate
2007-05-22
2007-05-22
O'Hara, Eileen B. (Department: 1646)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
C530S350000, C530S387100, C530S389100, C530S388850, C436S547000, C436S548000, C435S326000
Reexamination Certificate
active
10460594
ABSTRACT:
The present invention provides two novel polypeptides, referred to as the “N” and “C” fragments of hedgehog, or N-terminal and C-terminal fragments, respectively, which are derived after specific cleavage at a G↓CF site recognized by the autoproteolytic domain in the native protein. Also included are sterol-modified hedgehog polypeptides and functional fragments thereof. Methods of identifying compositions which affect hedgehog activity based on inhibition of cholesterol modification of hedgehog protein are described.
REFERENCES:
patent: 5143830 (1992-09-01), Holland et al.
patent: 5789543 (1998-08-01), Ingham et al.
patent: 5844079 (1998-12-01), Ingham et al.
patent: 0 020 029 (1980-12-01), None
patent: WO 96/16668 (1996-06-01), None
Farase et al., Cholesterol metabolism and embryogenesis, Trends in Genetics, 14(3): 115-120, Mar. 1998.
Kolf-Clauw et al., Abnormal cholesterol biosynthesis as a Smith-Lemli-Opitz syndrome disrupts normal skeletal development in the rat, J. Lab. Clin. Med., 131: 222-227, Mar. 1998.
Lee et al. Cell, vol. D71, pp. 33-50, Oct. 2, 1992.
Lange and Steck, “Cholesterol Homeostasis,”The Journal of Biological Chemistry, 269(47):29371-29374, The American Society for Biochemistry and Molecular Biology, Inc. (1994).
Porter et al., “Cholesterol Modification of Hedgehog Signaling Proteins in Animal Development,”Science, 274:255-259, American Association for the Advancement of Science (1996).
Porter et al., “Hedgehog Patterning Activity: Role of a Lipophilic Modification Mediated by the Carboxy-Terminal Autoprocessing Domain,”Cell, 86:21-34, Cell Press (1996).
Porter, Jeffery et al., “Hedgehog Patterning Activity: Role of a Lipophilic Modification Mediated by the Carboxy-Terminal Autoprocessing Domain,”Cell, vol. 86, Jul. 12, 1996, pp. 21-34.
Porter, Jeffery A. et al., “Cholesterol Modification of Hedgehog Signaling Proteins in Animal Development,”Science, vol. 274, Oct. 11, 1996, pp. 255-259.
Clarke et al., “Cellular lipid binding proteins: expression, function and nutritional regulation,”FESEB J., vol. 3, pp. 2480-2487, Nov. 1989.
Mohler et al., “Molecular organization and embryonic expression of the hedgehog gene involved in cell-cell communication in segmental patterning of Drosophila,”Development, vol. 115, pp. 957-971, 1992.
Lee et al., “Secretion and localized transcription suggest a role in positional signaling for products of the segmentation gene hedgehog”,Cell, vol. 71, pp. 33-50, Oct. 2, 1992.
Placzek, “Induction of floor plate differentiation by contact-dependent, homogenetic signals,”Development, vol. 117, pp. 205-218, 1993.
Krauss et al., “A Functionally Conserved Homolog of the Drosophila Segment Polarity Gene hh Is Expressed in Tissues with Polarizing Activity in Zebrafish Embryos”,Cell, vol. 75, pp. 1431-1444, Dec. 31, 1993.
Echelard, et al., “Sonic Hedgehog, a Member of a Family of Putative Signaling Molecules, Is Implicated in the Regulation of CNS Polarity,”Cell, vol. 75, pp. 1417-1430, Dec. 31, 1993.
Ngo, et al., “Computational Complexity, Protein Structure Prediction and Levinthal Paradox”,The Protein Folding Problem and Tertiary Structure Prediction, Merz, et al., eds., Birkhauser, Boston, pp. 433, 492-495, 1994.
Lee et al., “Autoproteolysis in hedgehog Protein Biogenesis”,Science, vol. 266, pp. 1528-1537, Dec. 2, 1994.
Tabata and Kornberg, “Hedgehog is a Signaling Protein with a Key Role in Patterning Drosophila Imaginal Discs”,Cell, vol. 76, pp. 89-102, Jan. 14, 1994.
Ingham, P.W., “Signaling by hedgehog family proteins in Drosophila and vertebrate development”,Cur. Opin. Gen. Dev., vol. 5, pp. 492-498, 1995.
Hynes, et al., “Induction of Midbrain dopamenergic Neurons by Sonic Hedgehog,”Neuron, vol. 15, pp. 35-44, Jul. 1995.
Beachy Philip A.
Porter Jeffrey A.
DLA Piper (US) LLP
O'Hara Eileen B.
The Johns Hopkins University School of Medicine
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