Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1992-06-19
1994-03-29
Chan, Y. Christina
Chemistry: molecular biology and microbiology
Spore forming or isolating process
5303879, 53038873, 5303873, 435 696, 4352523, C07K 1528, C12N 121, C12N 512, C12P 2108
Patent
active
052984206
ABSTRACT:
Membrane anchoring peptides are attached to the C terminal end of the heavy chain of the various immunoglobulin isotypes (IgM, IgD, IgA, IgE, or IgG). The membrane anchoring peptides span the cell membrane lipid bilayer of B cells thereby affixing the associated immunoglobulin to the cell membrane surface. The extracellular segments of these peptides are unique for different isotypes. Epitopes unique to the B cells which produce each isotype are formed, in whole or in part, by these extracellular segments. These membrane-bound immunoglobulin isotype-specific ("migis") extracellular epitopes are not present on the secreted, soluble form of the immunoglobulins, which are not bound to the cell surface by the membrane anchoring peptides. The antibodies of the invention (and other related products) specifically bind to the extracellular migis epitopes of human .mu. chain, human .delta. chain, or human .gamma. chain. The B cells which express the isotypes IgM, IgD or IgG are labeled for destruction when bound by such antibodies or products, and can be destroyed by the cytolytic or regulatory mechanisms of the immune system in order to cause immunosuppression.
REFERENCES:
patent: 5091313 (1992-02-01), Chang
Rincon et al. Immunology 1984, 53:713.
Sevier et al. Clinical Chem. 27(11) 1797-1806 1981.
Morrison et al. PNAS vol. 81 pp. 6851-6855 1984.
Cheng et al. Nature vol. 296 1982 p. 410.
Blattner et al. Nature vol. 307 p. 418 1984.
Riechmann et al. Nature 332:323-327 1988 (applicant's cited art).
Word et al. "The murine immunoglobulin .alpha. gene expresses multiple transcripts from a unique membrane exon" (particularly; p. 895, 2nd column) EMBO Journal 2:887-898 (1983).
Manning, D. D., "Heavy Chain Isotope Suppression: A Review of the Immunosuppressive Effects of Heterograms Anti-Ig Heavy Chain Antisera" J. Reticulo. Soc. 18:63-86 (1975).
Manning D. D. et al. "Suppression of Regionic Antibody (IgE) Formation in Mice by Treatment with Anti-U Antiserum" J. Exp. Med. 144:288-293 (1976). Manning
Bazin, H. et al. "Differential Effect of Neonatal Injections of Anti-U or Anti-.beta. Antibodies on the Synthesis of IgM, IgE, IgA, IgG1, IgG2a IgG2b and IgG2c Immunoglobulin Classes" J. Immunol. 121:2083-2087 (1978).
Tahghi Kilani, R. et al. "The Role of Humoral Immunity in Cryptospondium Spp. Intection Studies with B Cell-Depleted Mice" J. Immunol. 145:1571-1576 (1990).
Chan Y. Christina
Feisee L.
Mirabel Eric P.
Tanox Biosystems, Inc.
LandOfFree
Antibodies specific for isotype specific domains of human IgM an does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Antibodies specific for isotype specific domains of human IgM an, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Antibodies specific for isotype specific domains of human IgM an will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-790970