Antibodies specific for BCL-2 gene product

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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5303877, 5303879, 5303888, 5303897, 4241381, 4241551, 4241561, 435 7021, 4351722, 43524027, C07K 1618, A61K 39395, C12N 520

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055063446

ABSTRACT:
The sequence of the protein coding regions of the bcl-2 gene are provided as well as bacterial clones which produce the proteins. Assays are provided for detecting a class of B-cell neoplasms associated with a chromosome translocation between chromosomes 14 and 18. This translocation is involved in the majority of cases of human follicular lymphomas. One assay employs an antibody which is immunoreactive with a human protein which is over-expressed due to the chromosome translocation. Another assay involves measurement of the amount of mRNA which hybridizes to the gene proximal to the translocation break-point.

REFERENCES:
Tsujimoto et al., "Analysis of the Structure, Transcripts, and Protein Products of bcl-2, the Gene Involved in Human Follicular Lymphoma", Proc. Natl. Acad. Sci. USA 83:5214-5218 (1986).
Tsujimoto et al., "The t(14;18) Chromosome Translocations Involved in B-Cell Neoplasms Result from Mistakes in VDJ Joining", Science, 229:1390-1393 (1985).
Tsujimoto et al., "DNA Rearrangements in Human Follicular Lymphoma can Involve the 5' or the 3' region of the bcl-2 Gene", Proc. Natl. Acad. Sci. USA 84:1329-1331 (1987).
Tsujimoto et al., "Clustering of Breakpoints on Chromosome II in Human B-Cell Noeplasms with the t(11;14) Chromosome Translocation", Nature 315:340-343 (1985).
Tsujimoto et al., "Involvement of the bcl-2 Gene in Human Follicular Lymphoma", Science 228:1440-1443 (1985).
Tsujimoto et al., "Cloning of the Chromosome Breakpoint of Neoplastic B Cells with the t(14;18) Chromosome Translocation", Science 226:1097-1099 (1984).
Pegoraro et al., "A 14;18 and an 8;14 Chromosome Translocation in a Cell Line Derived from an Acute B-Cell Leukemia", Proc. Natl. Acad. Sci. USA 81:7166-7170 (1984).
Cleary et al., "Nucleotide Sequence of a t(14;18) Chromosomal Breakpoint in Follicular Lymphoma and Demonstration of a Breakpoint-Cluster Region Near a Transcriptionally Active Locus on Chromosome 18", Proc. Natl. Acad. Sci. USA 82:7439-7443 (1985).
Ngan et al., New England Journal of Medicine 318:1638-1644 (1988).
Reed et al., Nature 336: 259-261 (1988).

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