Antibodies or antigens bound to a macroporous hydrophobic synthe

Details Antibodies or antigens bound to a macroporous hydrophobic synthe Antibodies or antigens bound to a macroporous hydrophobic synthe

1990-06-14

1992-12-08

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 79, 435 793, 435 794, 435180, 436531, 436544, 436547, 436823, 436824, 530413, 530815, G01N 33535, G01N 33545, C07K 1708

Patent

active

051697572

ABSTRACT:
A device for immunological techniques is prepared containing a macroporous hydrophobic synthetic polymer cloth having antibodies or antigens directly adsorbed therein and directly absorbed and immobilized thereon. The cloth has a thickness of more than about 200 .mu.m and has spaces between fibres exceeding about 20 .mu.m in diameter, and preferably has a Frazier Air permeability, in CFM/ft.sup.2 at 0.5" H.sub.2 O of from about 215 to about 750 for thickness of from about 11 to about 40 mils such that it can accommodate a large volume of liquid per surface area, that it has a large surface area, and that it has minimum flow resistance. In immunoassays antibodies may be directly adsorbed therein and directly absorbed and immobilized thereon, and specific antigens from a selected test sample, may then be captured by the antibodies, to be detected conventionally. Alternatively antigens may be directly adsorbed therein and directly absorbed and immobilized thereon, and specific antibodies from a selected test sample, may be captured by the antigens, to be detected conventionally. This device can also be used for extraction and concentration of lipopolysaccharide antigens and for preparing biotinylated, affinity-purified antibodies.

REFERENCES:
patent: 4200690 (1980-04-01), Root et al.
patent: 4617326 (1986-10-01), Bjornberg et al.
patent: 4693985 (1987-09-01), Degen et al.
patent: 4808530 (1989-02-01), Means et al.
Morris, et al. Immunoassays in Food Analysis, Elsevier Applied Science Publishers, N.Y. 1985, pp. 153-171.
Yamazaki, et al., Biotechnology Letters, vol. 8, No. 2, 1986, pp. 107-110.

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