Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Reexamination Certificate
2005-10-04
2005-10-04
Navarro, Mark (Department: 1645)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
C530S387900, C435S975000
Reexamination Certificate
active
06951922
ABSTRACT:
Disclosed are novel synthetically-modifiedB. thuringiensischimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
REFERENCES:
patent: 5055294 (1991-10-01), Gilroy
patent: 5128130 (1992-07-01), Gilroy et al.
patent: 5349124 (1994-09-01), Fischhoff et al.
patent: 5380831 (1995-01-01), Adang et al.
patent: 5384253 (1995-01-01), Krzyzek et al.
patent: 5441884 (1995-08-01), Baum
patent: 5449681 (1995-09-01), Wickiser
patent: 5500365 (1996-03-01), Fischhoff et al.
patent: 5508264 (1996-04-01), Bradfisch et al.
patent: 5593881 (1997-01-01), Thompson et al.
patent: 0193259 (1986-09-01), None
patent: 0 228 838 (1986-12-01), None
patent: 0290395 (1988-11-01), None
patent: 0213818 (1991-02-01), None
patent: 0292435 (1995-07-01), None
patent: 0359472 (1995-12-01), None
patent: 0731170 (1996-09-01), None
patent: WO 84/02913 (1984-08-01), None
patent: WO93/07278 (1993-04-01), None
patent: WO95/02058 (1995-01-01), None
patent: WO95/06730 (1995-03-01), None
patent: WO95/30752 (1995-11-01), None
patent: WO95/30753 (1995-11-01), None
patent: WO 98/02039 (1998-01-01), None
Baum et al., “Novel Cloning Vectors forBacillus thuringiensis,” Appl. Environ. Microbiol., 56(11):3420-3428, 1990.
Bosch et al., “RecombinantBacillus thuringiensisCrystal Proteins with New Properties: Possibilities for Resistance Management,”Bio/Technology, 12:915-918, 1994.
Caramori et al., “In vivo generation of hybrids between twoBacillus thuringiensisinsect-toxin-encoding genes,”Gene, 98(1):37-44, 1991.
Caramori et al., “Bacillus thruingiensis kurstakihybrid endotoxin genes generated by In Vivo recombination,” ISBN 1-56081-028-9, 0(0):259-267, 1990.
Gill et al., “Identification, Isolation, and Cloning of aBacillus thuringiensisCryIAc Toxin-binding Protein from the Midgut of the Lepidopteran InsectHeliothis virescens,” J. Biol. Chem.270(45):27277-27282, 1995.
Grochulski et al., “Bacillus thuringiensisCryIA(a) Insecticidal Toxin: Crystal Structure and Channel Formation,”J. Mol. Biol.,254:447-464, 1995.
Honée et al., “The C-terminal domain of the toxic fragment of aBacillus thuringiensiscrystal protein determines receptor binding,”Mol. Microbiol., 5(11):2799-2806, 1991.
Knight et al., “Molecular Cloning of an Insect Aminopeptidase N that Serves as a Receptor forBacillus thuringiensisCryIA(c) Toxin,”J. Biol. Chem., 270(30):17765-17770, 1995.
Lee et al., “Domain III Exchanges ofBacillus thuringiensisCryIA toxins affect binding to different gypsy moth midgut receptors,”Biochem. Biophysical Research Communications, 216(1):306-312, 1995.
Masson et al., “The CryIA(c) Receptor Purified fromManduca sextaDisplays Multiple Specificities,”J. Biol. Chem., 270(35):20309-20315, 1995.
Mettus et al., “Expression ofBacillus thuringiensisδ Endotoxin Genes during Vegetative Growth,”Appl. Environ. Microbiol., 56(4):1128-1134, 1990.
Nakamura et al., “Construction of chimeric insecticidal proteins between the 130-kDa and 135-kDa proteins ofBacillus thuringiensissubsp.aizawaifor analysis of structure-function relationship,”Agric. Biol. Chem.,54(3):715-724, 1990.
Racapé et al., “Properties of the pores formed by parental and chimericBacillus thuringiensisinsecticidal toxins in planar lipid bilayer membranes,”Biophysical J.72(2) (part 2 of 2), A82, M-Pos329, 1997, ISSN: 0006-3495.
Raymond et al., “Larvicidal activity of chimericBacillus thuringiensisprotoxins,”Mol. Microbiol., 4(11):1967-1973, 1990.
Rudd et al., “Domain III Substitution inBacillus thuringiensisDelta-Endotoxin CryIA(b) Results in Superior Toxicity forSpodoptera exiguaand Altered Membrane Protein Recognition,”Appl. Environ. Microbiol.,62(5):1537-1543, 1996.
Rudd et al., “Different Domains ofBacillus thuringiensisδ-Endotixins Can Bind to Insect Midgut Membrane Proteins on Ligand Blots,”Appl. Environ. Microbiol., 62(8):2753-2757, 1996.
Schnepf et al., “Specificity-determining Regions of a Lepidopteran-specific Insecticidal Protein Produced byBacillus thuringiensis,”J. Biol. Chem.265(34):20923-20930, 1990.
Shadenkov et al., “Construction of a hybrid gene from CryIIIIA and CryIA(a) δ-endotoxin genes ofBacillus thuringiensisand expression of its derivatives inEscherichia colicells,”Mol. Biol.,27(4):586-591, Part 2, 1993.
Thompson et al., “Structure, Function and Engineering ofBacillus thuringiensisToxins,”Genetic Engineering, 17:99-117, 1995.
Vachon et al., “Mode of action ofBacillus thuringiensisinsecticidal crystal proteins: A study of chimeric toxins,”FASEB Journal10(3), A74, 429, 1996, ISSN: 0892-6638.
De Maagd et al., “Different domains ofBacillus thuringiensisδ-endotoxins can bind to insect midgut membrane proteins on ligand blots,”Applied and Environmental Microbiology, 62(8):2753-2757, 1996.
Honée et al., “A translation fusion product of two different insecticidal crystal protein genes ofBacillus thuringiensisexhibits an enlarged insecticidal spectrum,”Applied and Environmental Microbiology, 56(3):823-825, 1990.
International Search Report dated Apr. 20, 1998 (PCT/US97/21587)(MECO:205P).
Adang et al., “The reconstruction and expression of aBacillus thuringiensis cryIIIAgene in protoplasts and potato plants,”Plant Mol. Biol., 21:1131-1145, 1993.
Bernhard, “Studies on the delta-endotoxin ofBacillus thuringiensisvar.tenebrionis,” FEMS Microbiol. Letters, 33:261-265, 1986.
Herrnstadt et al., “A new strain ofBacillus thuringiensiswith activity against Coleopteran insects,”Bio/Technology, 4:305-308, 1986.
Höfte et al., “Structural and functional analysis of a cloned delta endotoxin ofBacillus thuringiensis berliner1715,”Eur. J. Biochem., 171:273-280, 1986.
Klier et al., “Cloning and expression of the crystal protein genes fromBacillus thuringiensisstrainberliner1715,”EMBO J., 1(7):791-799, 1982.
Koziel et al., “Field performance of elite transgenic maize plants expressing an insecticidal protein derived fromBacillus thuringiensis,”Bio/Technol., 11:194-200, 1993.
Krieg et al., “Bacillus thuringiensis var. tenebrionis, a new pathotype effective against larvae of Coleoptera,”Z. ang. Ent.,96:500-508, 1983.
Krieg et al., “New results onBacillus thuringiensis var. tenebrioniswith special regard to its effect on the Colorado beetle (Leptinotarsa decemlineata),”Anz. Schädlingskde Pflanzenschutz Umweltschutz, 57(8):145-150, 1984.
Murray et al., “Analysis of unstable RNA transcripts of insecticidal crystal protein genes ofBacillus thuringiensisin transgenic plants and electroporated protoplasts,”Plant Mol. Biol., 16:1035-1050, 1991.
Perlak et.al., “Genetically improved potatoes: protection from damage by Colorado potato beetles,”Plant Mol. Biol., 22:313-321, 1993.
Perlak et al., “Insect resistant cotton plants,”Bio/Technol., 8:939-943, 1990.
Perlak et al., “Modification of the coding sequence enhances plant expression of insect control protein genes,”Proc. Natl. Acad. Sci. USA, Biochem., 88:3324-3328, 1991.
Schnepf and Whiteley, “Cloning and expression of theBacillus thuringiensiscrystal protein gene inEscherichia coli,” Proc. Natl. Acad. Sci. USA, 78(5), 2893-2897, 1981.
Gill et al., Cytologic Activity and Immunological Similarity of theBacillus thuringiensissupsp.israelensisandBacillus thuringiensissubsp.morrisoniIsolate PG-14 Toxins.Appl. And Enviro. Microbio
Gilmer Amy Jelen
Malvar Thomas
Ball Timothy K.
Monsanto Technology LLC
Navarro Mark
LandOfFree
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