Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-09-02
1998-09-08
Stucker, Jeffrey
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 732, 435 792, 435 793, 435975, 436501, 436503, 436547, 436548, 436808, 5303871, 5303872, 53038822, 5303891, G01N 3353
Patent
active
058043934
ABSTRACT:
The present invention relates to an antibody which reacts specifically with the binding proteins for Bacillus thuringiensis .delta.-endotoxins and their derivatives. The invention further relates to an anti-idiotype antibody which reacts specifically with the binding proteins for Bacillus thuringiensis .delta.-endotoxins and their derivatives. The invention also provides a test kit which contains a novel antibody. The invention further relates to a method of isolating a novel B. thuringiensis .delta.-endotoxin, to said novel B. thuringiensis .delta.-endotoxin, to a method of identifying a novel B. thuringiensis .delta.-endotoxin which recognises a novel binding protein, to the novel B. thuringiensis .delta.-endotoxin, to a method of determining the amount of binding protein present, and to a method of reducing the resistance development potential in pest control. The invention further relates to the use of novel antibodies and test kits for analysing the immunological cross reactivity of toxin binding proteins to determine their degree of relationship and to determine the availability and accessibility of a binding protein for the corresponding toxin in different insects. The invention also relates to the use of the novel antibodies for analysing resistance which is attributable to the lack of the binding protein or of the binding sites.
REFERENCES:
Bradford, M.M., "A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding", Anal. Biochem., 72:248-254 (1976).
Caramori, T. et al., "In vivo Generation of Hybrids Between Two Bacillus thuringiensis Insect-toxin-encoding Genes", Gene, 98:37-44 (1991).
Delafield, F.P. et al., "Immunological Homology Between Crystal and Spore Protein of Bacillus thuringiensis", J. Bacteriol., 96(3):713-720 (1968).
Dixon, B., "B.T. Toxins Studied", Biotechnology, 9:415 (1991).
Garczynski, S.F., et al., "Identification of Putative Insect Brush Border Membrane-Binding Molecules Specific to Bacillus thuringiensis .delta.-Endotoxin by Protein Blot Analysis", Applied and Environ. Microbiol., 57(10):2816-2820 (1991).
Ge., A.Z., et al., "Location of the Bombyx mori Specificity Domain on a Bacillus thuringiensis .delta.-endotoxin Protein", PNAS, 86:4037-4041 (1989).
Geiser, M., et al., "The Hypervariable Region in the Genes Coding for Entomopathogenic Crystal Proteins of Bacillus thuringiensis: Nucleotide Sequence of the kurhdl Gene of subsp. kurstaki HD1", Gene, 48:109-118 (1986).
Haider, M.Z., et al., "Analysis of the Molecular Basis of Insecticidal Specificity of Bacillus thuringiensis Crystal .delta.-endotoxin", Biochem J., 248:197-201 (1987).
Harlow, E. et al., Antibodies, A Laboratory Manual, pp. 59-67, (Cold Spring Harbor Laboratory, 1988).
Hofte, H. et al., "Insecticidal Crystal Proteins of Bacillus thuringiensis", Microbiological Reviews, 53(2):242-255 (1989).
Huber-Lukac, M. et al., "Characterization of Monoclonal Antibodies to a Crystal Protein of Bacillus thuringiensis subsp. kurstaki", Infection and Immunity, 54(1):228-232 (1986).
Knowles, B.H. et al., "Characterization and Partial Purification of a Plasma Membrane Receptor for Bacillus thuringiensis var kurstaki Lepidopteran-specific .delta.-endotoxin", J. Cell Sci., 83:89-101 (1986).
Laemmli, J.K., "Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4", Nature, 227:680-685 (1970).
Madara, P.J., et al., "Affinity Purification of Polyclonal Antibodies from Antigen Immobilized in Situ in Sodium Dedecyl Sulfate-Polyacrylamide Gels", Anal. Biochem., 187:246-250 (1990).
Oddou, P., et al., "Identification and Characterization of Heliothis virescens Midgut Membrane Proteins Binding Bacillus thuringiensis .delta.-endotoxin", Eur. J. Biochem., 202:673-680 (1991).
Schnepf, H.E., et al., "The Amino Acid Sequence of a Crystal Protein from Bacillus thuringiensis Deduced from the DNA Base Sequence", J. Biol. Chem., 260(10):6264-6272 (1990).
Towbin, H., et al., "Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications", PNAS, 76(9):4350-4354 (1979).
Van Rie., J., et al., "Specificity of Bacillus thuringiensis .delta.-endotoxins: Importance of Specific Receptors on the Brush Border Membrane of the Mid-gut of Target Insects", Eur. J. Biochem., 186:239-247 (1989).
Van Rie, J., et al., "Mechanism of Insect Resistance to the Microbial Insecticide Bacillus thuringiensis", Science, 247:72-74 (1990).
Wolfersberger, M., et al., "Preparation and Partial Characterization of Amino Acid Transporting Brush Border Membrane Vesicles from the Larval Midgut of the Cabbage Butterfly (pieris brassicae)", Comp. Biochem. Physiol., 86A(2):301-308 (1987).
Geiser Martin
Hartman Herbert
Stock Pascale Oddou
Looper Valerie E.
Stucker Jeffrey
Thermo Trilogy Corporation
LandOfFree
Antibodies directed to the binding proteins of Bacillus thuringi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Antibodies directed to the binding proteins of Bacillus thuringi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Antibodies directed to the binding proteins of Bacillus thuringi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1281057