Antibodies and proteins useful for assaying virus infection in g

Details Antibodies and proteins useful for assaying virus infection in g Antibodies and proteins useful for assaying virus infection in g

1996-09-05

1999-10-12

Eisenschenk, Frank C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 71, 4352401, 5303883, 5303885, 530826, C12Q 170, G01N 3353, C12N 500, C07K 1600

Patent

active

059653558

ABSTRACT:
An approximately 37 kilodalton (kd) protein associated with grapevine leafroll disease infected plants is disclosed. The 37 kd protein is the coat protein for a grapevine leafroll-associated virus designated GLRaV-8. The grapevine virus-encoded 37 kd polypeptide is immunologically distinct from the approximately 36 kd proteins associated with GLRaV-4 or GLRaV-5 or the approximately 38 kd protein associated with GLRaV-1. The invention further provides a substantially pure antibody directed against the 37 kd virus-associated protein, a stable cell line capable of producing such a monoclonal antibody, and a method for assaying for a virus infection in Vitis species. The method involves detecting the presence of a 37 kd polypeptide encoded by an RNA-containing plant virus using an antibody that does not react with a viral encoded polypeptide of ca. 38 kd.

REFERENCES:
Boscia et al., Vitis 34(3) :171-175 (1995).
Boscia et al. (1990) Phytopathology 80:117.
Choueiri et al. Vitis, 35(2):91-93 (1996).
Gugerli et al. (1984) Rev Suisse Vitic. Arbotic. Hortic. 16:299-304.
Gugerli, P. and Ramel, M-E (1993) Extended Abstracts, 11th Meeting of the International Council for the Study of Viruses and Virus Diseases of the Grapvine, pp. 23-24.
Hu et al. (1990) J. Phytopathology 128:1-14.
Hu et al. (1990) Phytopathology 80:920-925.
Monis, Judit et al. Abstract (1997) Plant. Dis. 81:802-808.
Mullins et al., Biology of the Grapevine, Cambridge (1994).
Namba et al. (1991) Phytopathology 81:964-970.
"U.C. Davis Grapevine Virus Collection" (see Golino, D.A., (1992) Am. J. Enol. Vitic., 43:200-205); the Foundation Plant Material Service (FPMS) at the University of California, Davis, California.
Zee et al. (1987) Phytopathology 77, 1427-1434.
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Hu et al. Phytopathology 80:920-925, 1990.
Boscia et al. Phytopathology 80(1):117, 1990.
Pollini et al. J. Virol. Methods 429(1):107-116, 1993.
Golino, D.A. Am. J. Enol. Vitic., 43:200-205, 1992.
Gugerli et al. "Etiological Studies & Diagnostic of Grapevine Leafroll Disease Improved by Monoclonal Antibodies", In:Monoclonal Antibodies Magniculture: proceedings of the symposium "Perspectives for monoclonal antibodies in agriculture" Laboratory for Monoclonal Antibodies Wageningen, Netherlands, May 30, 1990 A. Schuts ed. pp. 47-54.

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