Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Reexamination Certificate
1998-02-09
2004-09-14
Nolan, Patrick J. (Department: 1644)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
Reexamination Certificate
active
06790940
ABSTRACT:
I. FIELD OF THE INVENTION
The present invention relates to antibodies which are active against a fusion polypeptide comprising a histidine portion, a process for the preparation thereof and their use.
II. BACKGROUND OF THE INVENTION
It is known to express a polypeptide in the form of a histidine fusion polypeptide. In such a polypeptide, a histidine portion of, e.g., 6-18 successive histidine residues is fused to the C- or N-terminus of the polypeptide. Hence it is possible to isolate the histidine fusion polypeptide by means of a nickel-chelate chromatographic column from the supernatant or cell lysate of the cell expressing it.
However, the above column is expensive. Furthermore, its use costs a lot of time. Therefore, it is not suited for the rapid detection of the expression of a histidine fusion polypeptide. But such a detection is necessary, particularly when it is the objective to screen large numbers of cells.
Thus, it is the object of the present invention to provide means by which the expression of a histidine fusion polypeptide can be detected rapidly.
III. SUMMARY OF THE INVENTION
The present invention relates to an antibody active against a fusion polypeptide comprising a histidine portion, a process for the preparation thereof and its use.
REFERENCES:
patent: 5840834 (1998-11-01), Peterson et al.
patent: A43 39 533 (1995-06-01), None
patent: C195 07 166 (1996-04-01), None
patent: WOA94 08241 (1994-04-01), None
Cappel Catalog #57041, Apr. 1999.*
Clinical Chemistry, vol. 27, 1797-1806, 1981.*
Janssen et al. J.B.C. vol. 270: 11222-11229, 1995.*
Randall et al. Vaccine et al., vol. 11, pp. 1247-1252, 1993.
Campbell et al., 1993, “Functional Complementation of anEscherichia ColiRibonuclease H Mutation by a Cloned Genomic Fragment from the TrypanosomatidCrithidia Fasciculata”, Proc. Natl. Acad. Sci. U.S.A. 90:9350-9354.
Evans et al., 1992, “Immunodetection of Recombinant Proteins Based on Antibodies Directed Against a Metal Binding Peptide Engineered for Purification by Immobilized Metal Affinity Chromatography”,Journal of Immunological Methods 156:231-238.
Patel et al., 1995, “The Product of the UL6 Gene of Herpes Simplex Virus Type 1 is Associated with Virus Capsids”,Virology 206:465-478.
Pederson et al., 1994, “Molecular Characterization of the AL3 Protein Encoded by a Bipartite Geminivirus”,Virology 202:1070-1075.
Zentgraf et al., 1995, “Detection of Histidine-Tagged Fusion Proteins by Using a High-Specific Mouse Monoclonal Anti-Histidine Tag Antibody”,Nucleic Acids Res. 23(16):3347-3348.
Frey Manfred
Schwinn Susanne
Tessmer Claudia
Velhagen Iris
Zentgraf Hanswalter
Deutsches Krebsforschungszentrum Stiftung des offentlichen Recht
Dorsey & Whitney LLP
Millauer Birgit
Nolan Patrick J.
LandOfFree
Antibodies active against a fusion polypeptide comprising a... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Antibodies active against a fusion polypeptide comprising a..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Antibodies active against a fusion polypeptide comprising a... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3240625