Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2006-05-23
2006-05-23
Shukla, Ram R. (Department: 1633)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C536S023700, C435S320100, C435S233000, C435S252330
Reexamination Certificate
active
07049097
ABSTRACT:
An antibiotic-independent vector for stable vector maintenance and high protein expression and a method for gene expression using this vector are disclosed. The stable maintenance of the vector is due to expression of a glutamic acid racemase to complement a D-glutamic acid auxotroph. No antibiotics, such as ampicillin, are necessary for the stable maintenance of this vector.
REFERENCES:
patent: 5919663 (1999-07-01), Brey et al.
patent: 0231919 (1999-01-01), None
Ferrari E, Henner DJ, Yang MY, Isolation fo an alanine racemase gene romBacilus subtilisand its use for plasmid maintenance inB. subtilis, 1985, Bio/Technology 3:1003-1007.
Galkin A, Kulakova L, Kurihara T, Yoshimura T, Esaki N, Proteins from cold-adapted bacteria: evolutionary and structural relationships with mesophilic and thermophilic counterparts, May 2000, Genbank Accession No. AF263927.
Petty KJ, Metal-chelate affinity chromatography, in Current Protocols in Molecular Biology, Wiley and Sons, 1996, pp 10.11.10-10.11.24.
Choi Yoon Ho
Hong Seung Pyo
Lee Seung Goo
Poo Ha Ryoung
Sung Moon Hee
Bioleaders Corporation
Korea Research Institute of Bioscience and Biotechnology
Riggins Patrick S.
Shukla Ram R.
Staas & Halsey , LLP
LandOfFree
Antibiotics-independent vector for constant high-expression... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Antibiotics-independent vector for constant high-expression..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Antibiotics-independent vector for constant high-expression... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3595449