Antibiotic YI-HU3

Details Antibiotic YI-HU3 Antibiotic YI-HU3

1988-08-15

1990-07-10

Lilling, Herbert J.

Drug, bio-affecting and body treating compositions

Fermentate of unknown chemical structure

4352533, 435874, A61K 3574, C12R 138, C07G 1100, C12P 104

Patent

active

049405829

DESCRIPTION:

BRIEF SUMMARY
FIELD OF THE INVENTION

This invention relates to a novel antibiotic. More particularly, the invention relates to novel antibiotic YI-HU3 possessing antimicrobial activity and antitumour activity as well as the process for preparing the same.


TECHNOLOGICAL BACKGROUND

Conventionally, various physiologically active substances which are produced by microorganisms are known in the art. Some of them are used as medicines.
There still exists, however, a desire for the development of -physiologically active substances which are more useful as medicines or agrochemicals.


DISCLOSURE OF THE INVENTION

The present inventors have isolated a number of microorganisms from natural soils and undertaken extensive studies on the product produced by such microorganisms. As a result, the inventors discovered that a strain separated from a soil at Wakayama Prefecture, Japan is capable of producing novel antibiotic YI-HU3 having antimicrobial and antitumour activities. The discovery has led to the completion of this invention.
Accordingly, an object of this invention is to provide novel antibiotic YI-HU3 and the process for preparing the same.


BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the ultraviolet absorption spectrum of antibiotic YI-HU3 of this invention, and FIGS. 2, 3 and 4 are the infrared spectrum, .sup.1 H--NMR spectrum, and .sup.13 C--NMR spectrum, respectively, of the same substance.


THE BEST MODE FOR PRACTICING THE INVENTION

The strain YI-HU which is a typical microorganism producing the antibiotic YI-HU3 of this invention has the following characteristics:
(1) Morphology
Following characteristics were confirmed by the light or electron microscope observations of the microorganism of the YI-HU strain cultured in a potato-dextrose slant culture medium at 28.degree. C. for 3 days.
The cells had a size of 1-5.mu. with no polymorphism. They had flagella and were mobile. No sporogenesis was observed. The Gram staining was negative and the strain had no acid fastness.
(2) Growth characteristics on various culture media:
The growth characteristics of the YI-HU strain on various culture media, as observed on the microorganisms cultured at 28.degree. C. for 3 days, are as follows:
ar plate culturing Color diffusion into the medium was not observed. thin film being formed.
(3) Physiological Characteristics


______________________________________ Nitrate reduction negative Indole production negative Starch hydrolysis negative Citric acid utilization positive Colored substance formation positive Urease negative Oxidase positive Reaction to oxygen aerobic Esculin hydrolysis negative Lysine decarbonation reaction positive Arginine decomposition negative Ornithine decarbonation reaction negative Acylamylase negative ______________________________________ medium for 24 hours.


______________________________________ Temperature (.degree.C.) Growth ______________________________________ 4 - 25 + 30 + 40 + 45 - ______________________________________ 30.degree. C. for 7 days)


______________________________________ Sugar Conditions Growth ______________________________________ Glucose closed - open + ______________________________________


______________________________________ Sugar Growth ______________________________________ Xylose + Glucose + Fructose + Galactose + Maltose - Sucrose - Lactose - Mannitol + Starch + ______________________________________
Based on the above mycological characteristics, especially based on the fact that the microorganism (i) is a Gram-negative rod, (ii) is oxidase positive, (iii) has flagella and is mobile, (iv) is aerobic, and (v) is not sporogenous, the YI-HU strain is judged to be a strain belonging to Pseudomonas.
Further, according to the retrieval from Bergey's Manual of Determinative Bacteriology (8th edition) based on the above characteristics, Pseudomonas gladioli and Pseudomonas cepacia are detected as relatives of the YI-HU strain. Pseudomonas cepacia, however, is differen

REFERENCES:
patent: 4062943 (1977-12-01), Lindberg

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