Antibiotic detection method

Details Antibiotic detection method Antibiotic detection method

1978-06-12

1980-12-16

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

424 1, G01N 3114

Patent

active

042398525

ABSTRACT:
A process for rapidly detecting as little as 0.001 I.U. ml of antibiotic in a sample of liquid such as milk. The process comprises the steps of incubating the sample together with a tagged antibiotic or antibiotic precursor and antibiotic sensitive cells under conditions which allow antibiotic molecules to attach to receptor sites in or on the cells, separating the cells with immobilized antibiotic from the remainder of the reaction mixture, and determining the quantity of tagged antibiotic on the cells. The amount of tagged antibiotic on the cells is a function of the quantity of antibiotic present in the sample. The process is well suited for detecting penicillin-type antibiotics and may be practiced using peroxidase-tagged 6-amino penicillanic acid and sonicated, penicillin supersensitive Bacillus stearothermophilus immobilized on a support.

REFERENCES:
patent: 3673410 (1972-06-01), Waite et al.
patent: 3817837 (1974-06-01), Rubenstein et al.
Edwards et al., Journal of Bacteriology, Aug. 1969 pp. 459-462.
Blumberg et al., Bacteriological Reviews, vol. 38, No. 3, pp. 291-335 (Sep. 1974).
Spratt, Eur. J. Biochem. vol. 72, pp. 341-352 (1977).

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