Antibiotic detection method

Details Antibiotic detection method Antibiotic detection method

1978-11-22

1980-12-16

Padgett, Benjamin R.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 424 12, 422 61, G01N 3316, A61K 4300, B65D 8132

Patent

active

042397456

ABSTRACT:
A process for rapidly detecting as little as 0.001 I.U./ml of antibiotic in a sample of liquid such as milk. The process comprises the steps of incubating the sample together with a tagged antibiotic or antibiotic precursor and antibiotic sensitive cells under conditions which allow antibiotic molecules to attach to receptor sites in or on the cells, separating the cells with immobilized antibiotic from the remainder of the reaction mixture, and determining the quantity of tagged antibiotic on the cells. The amount of tagged antibiotic on the cells is a function of the quantity of antibiotic present in the sample.

REFERENCES:
patent: 3673410 (1972-06-01), Waite et al.
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3852157 (1974-12-01), Rubenstein et al.
Edwards et al., Journal of Bacteriology, vol. 99, No. 2, 1969, pp. 459-462.
Blumberg et al., Bacteriological Reviews, vol. 38, No. 3, 1974, pp. 291-335.
Spratt, Evr. J. Biochem., vol. 72, 1977, pp. 341-352.

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