Drug – bio-affecting and body treating compositions – Fermentate of unknown chemical structure – Having a known elemental analysis
Patent
1982-07-13
1984-12-11
Goldberg, Jerome D.
Drug, bio-affecting and body treating compositions
Fermentate of unknown chemical structure
Having a known elemental analysis
435169, A61K 3574, C12P 106
Patent
active
044877625
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
The present invention relates to a new antibiotic 5057B substance and a process for production thereof.
DESCRIPTION OF THE PRIOR ART
The present inventors previously found that an actinomycete Streptomyces sp. 5057 strain belonging to the genus Streptomyces (FERM BP-62) produces a substance having an antibacterial activity in its culture broth and isolated an antibiotic 5057(A) from the culture broth (refer to the specification of Japanese Patent Publication No. 8641/1979).
The present inventors, in the course of further researches, have found that a new substance different from the 5057A substance is present in said culture broth and isolated the same, which was designated as the 5057B substance.
The 5057B substance, in view of its physicochemical and biological properties, is an antibiotic belonging to the antibiotic group of polyether series. Among antibiotics of polyether series, there are heretofore known, as those showing ultraviolet absorption in the vicinity of 290 nm, lysocellin (The Journal of Antibiotics, Vol. 28, Pages 118-121, 1975) and the 5057A substance (Japanese Patent Publication No. 8641/1979); however, the 5057B substance is a new antibiotic which differs from the above-mentioned known antibiotics in the melting point, specific rotation, infrared absorption spectrum, and the Rf value on silica gel thin layer chromatography.
DISCLOSURE OF THE INVENTION
The present invention relates to a new antibiotic 5057B substance having a potent antibacterial activity, especially against gram-positive bacteria, and a process for production thereof comprising cultivating a microoragnism, belonging to the genus Streptomyces, which has the ability to produce the 5057B substance and then recovering the 5057B substance from the culture broth.
The strain Streptomyces sp. 5057, belonging to the genus Streptomyces, employed in the production of the 5057B substance was deposited on May 1, 1981 with Fermentation Research Institute, Agency of Industrial Science and Technology (No. 1-3, Yatabechohigashi 1-chome, Tsukuba District, Ibaragi Prefecture, Japan) and accorded the accession number FERM BP-62. Microbiological properties of the strain is described in detail in the specification of Japanese Patent Publication No. 8641/1979.
In order to obtain the 5057B substance according to the present invention, ordinary methods for cultivation of actinomycetes can be employed, but a cultivation with aeration-agitation is suited for the industrial production. The cultivation temperature of 25.degree.-35.degree. C. is usual, but the temperature of 30.degree. C. is preferable. As a culture medium, there can be used one customarily employed for cultivation of microoragnisms belonging to the genus Streptomyces, for example, one containing carbon sources such as glucose, starch, glycerol, dextrin, sucrose, and animal or vegetable oils and nitrogen sources such as soybean meal, corn steep liquor, wheat embryo, and ammonia. Further, if necessary, inorganic salts such as calcium carbonate, sodium chloride, potassium chloride, and phosphates can be added; it is also possible to suitably add organic or inorganic salts having an action to assist the growth of the microoragnism and encourage the production of the 5057B substance. The accumulation of the 5057B substance produced reaches the maximum usually 4-8 days after the start of the cultivation in both cases of shaking and tank cultures.
The 5057B substance is recovered from the culture broth by utilizing its physiochemical properties; since the substance is lipo-soluble and acidic, there can be used, in a suitable combination, extraction with a variety of organic solvents, chromatography on a variety of activated adsorbents, and other methods. For example, an isolation method consisting of a combination of extraction and chromatography is carried out as follows: The culture broth, after addition of a filter aid such as diatomaceous earth and Radiolite 700 and the like, is filtered, and the filtrate and the microbial cells are each extracted with su
Taku Mizuno
Yoko Kusakabe
Goldberg Jerome D.
Kaken Chemical Co. Ltd.
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