Anti-sense RNA for CAB transcript to reduce chlorophyll content

Details Anti-sense RNA for CAB transcript to reduce chlorophyll content Anti-sense RNA for CAB transcript to reduce chlorophyll content

1995-12-12

1998-06-30

Robinson, Douglas W.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

536 231, 536 245, 435 911, 435 913, 4353201, C12N 1529, C12N 1582, A01H 400, A01H 500

Patent

active

057736928

ABSTRACT:
The initial steps in photosynthesis are the conversion of light energy into chemical energy. This conversion is performed by the multisubunit protein-pigment complexes of the thylakoid membranes. Oxygen-evolving photosystems contain photosystem I (PSI) and photosystem II (PSII), which act in tandem. In PSII, the majority of light-adsorbing chlorophylls are attached to LHCII, the light harvesting complex associated with this photosystem. LHCII is the most abundant member of the family of chlorophyll a/b binding (CAB) proteins. A gene encoding a type I chlorophyll a/b binding protein of LHCII (ICABPSII) has been cloned from Brassica napus L. An anti-sense RNA of this gene has been used to reduce the amount of chlorophyll a/b binding protein and thus reduce the amount of chlorophyll in the resulting transgenic plants. By using "site" specific promoters the reduction of chlorophyll can be targeted to specific organelles in the transgenic plant and thus can be used to reduce the green color at these sites. Thus it is possible to use the anti-sense RNA of a chlorophyll a/b binding protein as a means for degreening, for example fruits, seeds or floral parts in transgenic plants.

REFERENCES:
Boivin et al. Genome vol. 36, pp. 139-146, 1993. Isolation of Lhcb3 seuences from Brassica napus: evidence for conserved genes encoding LHCII type III chloropyll a/b binding proteins.
Pichersky et al. Plant Molecular Biology 9: 205-216, 1987. Molecular characterization and genetic mapping of DNA sequences encoding the Type I chlorophyll a/b binding polypeptide of photosystem I in Lycopersicon esculentum (tomato).
Cashmore, Anthony R., Structure and expression of a pea nuclear gene encoding a chlorophyll a/b binding polypeptide. Proc. Natl. Acad. Sci., USA. vol. 81, pp. 2960-2964, May (1984) Biochemistry.
Flachmann, Ralf et al., Accumulation of Plant Antenna Complexes is Regulated by Post-Transcriptional Mechanisms in Tobacco. The Plant Cell, vol. 7, pp. 149-160, Feb. (1995).
Politeski, J.C. et al., Reducing Chlorophyll Accumulation in Transgenic Brassic Napus Seeds. Supplement to Plant Physiology, vol. 105, No. 1, May (1994).
Johnson-Flanagan, A.M. et al., Degreening in Canola (Brassica napus, cv. Westar) Embryos under Optimum Conditions. J. Plant Physiol. vol. 136, pp. 180-186, (1990).
Johnson-Flanagan, A.M. et al., The Impact of Sublethal Freezing during Maturation on Pigment Content in Seeds of Brassica napus. J. Plant Physiol. vol. 136, pp. 385-390, (1990).
Johnson-Flanagan, A.M. et al., A Method to Study Seed Degreening Using Haploid Embryos of Brassica napus cv. Topas. J. Plant Physiol. vol. 141, pp. 487-493, (1993).
Johnson-Flanagan, A.M. et al., Peroxidase-mediated chlorophyll bleaching in degreening canola (Brassica napus) seeds and its inhibition by sublethal freezing. Physiologia Plantarum 80, pp. 453-459, Copenhagen (1990).
Johnson-Flanagan, A.M. et al., The impact of freezing during maturation on storage products in canola seeds. Physiologia Plantarum, 81:301-308 (1991) .

Affiliated with

Also associated with

Rating

Anti-sense RNA for CAB transcript to reduce chlorophyll content does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Anti-sense RNA for CAB transcript to reduce chlorophyll content , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Anti-sense RNA for CAB transcript to reduce chlorophyll content will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1861716