Anti-platelet immunoglobulin bead positive control

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

Reexamination Certificate

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C435S006120, C435S007100, C435S007200, C435S007920, C435S007930, C435S007940, C435S007950, C435S967000, C435S973000, C436S010000, C436S063000, C436S501000, C436S506000, C436S507000, C436S509000, C436S513000, C436S518000, C436S519000, C436S523000, C436S524000, C436S527000, C436S528000, C436S531000, C436S533000, C436S534000, C436S536000, C436S538000, C436S546000, C436S805000, C436S811000

Reexamination Certificate

active

06951716

ABSTRACT:
Immunoassay methods and apparatus are provided which utilize flow cytometry, coated latex microspheres, and fluorochrome labeled antibodies, to simultaneously detect the presence and amount of one or more analytes in a sample. Beads of several different sizes, colors or shapes, each bead are coated with a different analyte, for the simultaneous detection of one or more analytes and of cell components. The invention is also directed to platelet Ig positive control reagents and assays which provide for the setting of the fluorescence positive region for each patient. The platelet control is sized to fit between the platelets and red cells and thus making it ideal as a true biological control.

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patent: 6280618 (2001-08-01), Watkins et al.
patent: 2003/0008410 (2003-01-01), Hechinger
patent: 2003/0194818 (2003-10-01), Hechinger
Greenwalt et al. An enzyme linked antiglobulin test to quantify nanogram quantities of IgG on polystyrene microspheres. Vox Sang. 63 (4) pp. 272-275 (1992).
Saunders et al. Amplified flow-cytometric separation-free fluorescence immunoassays. Clin. Chem. 31 (12) pp. 2020-2023 (1985).
Fluwyler et al. Flow Microsphere Immunoassay for the Quantitative and Simultaneous Detection of Multiple Soulble Analytes. Methods in Cell Biology. vol. 33. pp. 613-629 (1990).

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