Anti-octopus rhodopsin monoclonal antibody

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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5303882, 43524027, 4351722, 435 7021, C07K 1528, C12N 520, C12N 1502, C12P 2108

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054140722

ABSTRACT:
The invention relates to a monoclonal antibody which binds to octopus rhodopsin and octopus metarhodopsin wherein said monoclonal antibody binds with higher affinity to metarhodopsin than to rhodopsin and does not inhibit rhodopsin-metarhodopsin interconversion upon exposure to blue or red light. The monoclonal antibody binds to a peptide having the sequence Val-Ile-Gly-Arg-Pro-Met-Ala-Ala-Ser-Lys-Lys-Met-Ser. The photochemical reaction of rhodopsin thin layers may be detected using the anti-rhodopsin monoclonal antibodies of the invention.

REFERENCES:
patent: 4444878 (1984-04-01), Paulus
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Journal of Neutrocytology 12, 785-803 (1983) The Subcellular Localization of Rat Photoreceptor-Specific Antigens Donna M. Fekete & Colin J. Barnstable.
Biochemistry 1984, 23, 6544-6549 Localization of Binding Sites for Carboxyl Terminal Specific AntiRhodopsin Monoclonal Antibodies Using Synthetic Peptides Donald Mackenzie, Anatol Arendt, Paul Hargrave, J. Hugh McDowell & Robert S. Molday.
J. Cell. Biol. vol. 105 (5 pt.2), 1985, p. 222a, abstract No. 843; L. J. Robles et al.: "Monoclonal antibody localization of opsin in squid photoreceptors".

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