Anti-metasin antibodies and their methods of use

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Reexamination Certificate

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C530S387900, C424S139100, C424S141100, C424S145100

Reexamination Certificate

active

07112662

ABSTRACT:
The present invention aims at providing an antibody, by which metastin or its derivative can be quantified specifically with a high sensitivity, a method of detecting/quantifying metastin or its derivative using the antibody, and a diagnostic agent (e.g., a diagnostic for pregnancy) the same.Specifically, an antibody capable of specifically reacting with a partial peptide at the N-terminus or C-terminus of a polypeptide having the amino acid sequence represented by SEQ ID NO: 1 or its derivative, and a method of detecting/quantifying metastin or its derivative using the antibody as well as a diagnostic agent using the same.

REFERENCES:
patent: 1 126 028 (2001-08-01), None
Muir et al., “AXOR12, a Novel Human G Protein-coupled Receptor, Activated by the Peptide KiSS-1”, The Journal of Biological Chemistry 276 (31):28969-28975 (2001).
Ohtaki et al., “Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein-coupled receptor”, Nature 411:613-617 (2001).
Hori et al., “Metastin Suppresses the Motility and Growth of CHO Cells Transfected with its Receptor”, Biochemical and Biophysical Research Communications, 286:958-963 (2001).
West et al., “Chromosome Localization and Genomic Structure of the KiSS-1 Metastasis Suppressor Gene (KISS1)”, Genomics, 54:145-148 (1998).
Kotani et al., “The Metastasis Suppressor Gene KiSS-1 Encodes Kisspeptins, the Natural Ligands of the Orphan G. Protein-coupled Receptor GPR54”, The Journal of Biological Chemistry, 276(37):34631-34636 (0001).
Lee et al., “KiSS-1, a Novel Human Malignant Melanoma Metastasis-Suppressor Gene”, Journal of the National Cancer Institute, 88(23): 1731-1737)1996).
Welch et al., “Molecular biology of breast cancer metastasis Genetic regulation of human breast carcinoma metastasis”, Breast Cancer Res., 2:408-416 (2000).
Lee et al., “Suppression of Metastasis in Human Breast Carcinoma MDA-MB-435 Cells after Transfection with the Metastasis Suppressor Gene, KiSS-1”, Cancer Research 57:2384-2387 (1997).

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