Anti-invasion factor containing cultures

Details Anti-invasion factor containing cultures Anti-invasion factor containing cultures

1980-04-22

1982-10-26

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435240, 424 95, C12N 500, C12P 2100

Patent

active

043562612

ABSTRACT:
Chondrocytes are cultured to produce cartilage tissue from which an anti-invasion factor (AIF) may be recovered. Mammalian cartilage providing at least about 60% viable chondrocytes is enzymatically digested to denude the cells of their extracellular matrix. The denuded cells are plated at high density and cultured to produce cartilage tissue.

REFERENCES:
patent: Re30239 (1980-03-01), Kuettner et al.
patent: 3450598 (1969-06-01), Welsh et al.
patent: 4176177 (1979-11-01), Kuettner
Muller et al., Exp. Cell Res., 108, 47-55, (1977).
Klagsbrun, Methods in Enzymology, LVIII, Academic Press, New York, 560-564, (1979).
O. W. Wiebkin and H. Muir, FEBS Letters, vol. 37, No. 1, (1973), pp. 42-46.

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