Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
1998-11-25
2001-10-23
Huff, Sheela (Department: 1642)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S004000, C435S007720, C435S325000, C435S326000, C435S327000, C435S330000, C435S331000, C435S007920, C530S350000, C530S351000, C530S387100, C530S387700, C530S387900
Reexamination Certificate
active
06306608
ABSTRACT:
TECHNICAL FIELD
This invention relates to antibodies which react with a new protein, human LECT2, hybridomas for producing the antibodies, and a method and a kit for measuring the human LECT2.
BACKGROUND ART
Recently, neutrophils became known to participate in the damage of cancer cells. As some cancer tissues are observed to have been infiltrated with neutrophils, it is believed that neutrophils respond to chemotactic factors secreted from cancer cells.
LECT2 (Leukocyte-derived chemotaxin 2) was discovered during the search for such chemotactic factors secreted from cancer cells, and this seems to be a chemotactic factor which has been obtained from a culture supernatant of T-cell leukemia cell SKW-3.
Human LECT2 was newly discovered as a protein having equal to or more than 90% homology to bovine LECT2 based on human cDNA libraries, using DNA coding LECT2 in bovine serum which is included in a culture supernatant of T-cell leukemia cell SKW-3. From the following Table 1, the amino acid sequence of the human LECT2 can be compared with that of the bovine LECT2.
TABLE 1
1 5 10 15
SEQ ID NO:1
HUMAN LECT2
Met Phe Ser Thr Lys Ala Leu Leu Leu Ala Gly Leu Ile Ser Thr
BOVINE LECT2
20 25 30
SEQ ID NO:9
HUMAN LECT2
Ala Leu Ala Gly Pro Trp Ala
Asn
Ile Cys Ala Gly Lys Ser Ser
BOVINE LECT2
Gly Pro Trp Ala
Ile
Ile Cys Ala Gly Lys Ser Ser
SEQ ID No: 9
35 40 45
HUMAN LECT2
Asn Glu Ile Arg Thr Cys Asp
Arg
His Gly Cys Gly Gln Tyr Ser
BOVINE LECT2
Asn Glu Ile Arg Thr Cys Asp
Gly
His Gly Cys Gly Gln Tyr Thr
50 55 60
HUMAN LECT2
Ala Gln Arg
Ser
Gln
Arg Pro
His Gln Gly Val Asp Val Leu Cys
BOVINE LECT2
Ala Gln Arg
Asn
Gln
Lys Leu
His Gln Gly Val Asp Val Leu Cys
65 70 75
HUMAN LECT2
Ser
Ala
Gly Ser Thr Val Tyr Ala Pro Phe Thr Gly Met Ile
Val
BOVINE LECT2
Ser
Asp
Gly Ser Thr Val Tyr Ala Pro Phe Thr Gly Ile
Met
80 85 90
HUMAN LECT2
Gly Gln Glu Lys Pro Tyr
Gln
Asn Lys Asn Ala Ile Asn Asn Gly
BOVINE LECT2
Gly Gln Glu Lys Pro Tyr
Lys
Asn
95 100 105
HUMAN LECT2
Val Arg Ile Ser Gly
Arg
Gly Phe Cys
Val
Lys Met Phe Tyr Ile
BOVINE LECT2
Ile Ser Gly
Gly
Gly Phe Cys Ile Lys
110 115 120
HUMAN LECT2
Lys Pro Ile Lys Tyr Lys Gly
Pro
Ile Lys Lys Gly Glu Lys Leu
BOVINE LECT2
Tyr Lys Gly
Ser
Ile
125 130 135
HUMAN LECT2
Gly Thr Leu Leu Pro Leu Gln Lys Val Tyr Pro Gly Ile Gln Ser
BOVINE LECT2
Val Tyr Pro Gly Ile Gln Ser
140 145 150
(SEQ ID NO:9)
HUMAN LECT2
His
Val
His Ile Glu Asn Cys Asp
Ser
Ser Asp Pro Thr Ala Tyr
BOVINE LECT2
His
Ile
His Ile Glu Asn Cys Asp
Leu
Ser Asp Pro Thr
151
HUMAN LECT2
Leu
(SEQ ID NO: 1)
BOVINE LECT2
Since the human LECT2 is believed to be a chemotactic factor similar to the bovine LECT2 and its applications to grasping disease conditions and to treatment of cancer are expected, a method for measuring the human LECT2 has been desired to be established.
At first, the bovine LECT2 and the human LECT2 used to be called LECT2a and LECT2b, respectively. However, these names were thought to be inappropriate and therefore changed.
The present invention is presented in respect of the aforementioned background, aiming to provide antibodies against human LECT2, cells for producing them and a method and a kit for measuring the human LECT2.
SUMMARY OF THE INVENTION
In order to solve the aforementioned problem, the antibodies of the first invention specifically react with human LECT2 (a protein having an amino acid sequence indicated in SEQ ID NO: 1 in the sequence table). Such antibodies include, for example, an antibody produced by hybridoma clone G2A5D7 (Accession No. FERM P-15638), an antibody produced by hybridoma clone A1G1C6 (Accession No. FERM P-15639), an antibody produced by hybridoma clone 5C5 (Accession No. FREM P-15640), an antibody produced by hybridoma clone H12D10D6 (Accession No. FERM P-15641) and an antibody produced by hybridoma clone 89F2 (Accession No. FERM P-16229).
The hybridomas of the second invention are characterized by their production of the antibodies which specifically react with the human LECT2. Such hybridomas include, for example, hybridoma clones G2A5D7 (Accession No. FERM P-15638), A1G1C6 (Accession No. FERM P-15639), 5C5 (Accession No. FERM P-15640), H12D10D6 (Accession No. FERM P-15641) and 89F2 (Accession No. FERM P-16229).
A measuring method of the human LECT2 of the third invention characteristically includes the following procedures from a) to c) and from d) to f):
a) The human LECT2 as a standard material is reacted with an immobilized antibody obtained by affixing a first antibody which specifically reacts with the human LECT2 to an insoluble support medium;
b) then, the product is reacted with a labeled antibody obtained by labeling a
Davis & Bujold P.L.L.C.
Harris Alana M.
Huff Sheela
Medical & Biological Laboratories Co. Ltd.
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